Protein Purification

Supplier: Cytiva

CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

Supplier: Abcam

Protein A Agarose (High Affinity) (ab193255) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Supplier: Abcam

The Antibody Concentration and Clean Up Kit for Latex & Europium kit (ab269965) removes small molecule buffer components from the antibody or protein prior to use in the conjugation reaction.

Supplier: Cytiva

Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns such as Superdex, Superose, and Sephacryl.

Supplier: Cytiva

Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media.

Supplier: Cytiva

Calmodulin Sepharose™ 4B is calmodulin immobilised by the CNBr method to Sepharose™ 4B. Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells. This protein is involved in many cellular processes such as glycogen metabolism, cytoskeletal control, neurotransmission, phosphate activity and control of NAD⁺/NADP⁺.

Supplier: Cytiva

Sephacryl High Resolution gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

Supplier: Thermo Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Supplier: Thermo Scientific

Thermo Scientific Pierce Glutathione Superflow Agarose consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose that is designed for high-purity, large-scale purification of GST-tagged proteins.

Supplier: Thermo Scientific

Thermo Scientific Pierce Avidin Agarose can be used in a variety of applications for the affinity purification of biotinylated macromolecules.

Supplier: Thermo Scientific

Thermo Scientific SulfoLink Immobilization Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Supplier: Thermo Scientific

Pierce F(ab')2 Micro Preparation Kit uses immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.

Supplier: Thermo Scientific

The AminoLink Immobilization Kit contains AminoLink Coupling Resin and associated reagents sufficient to perform 5 protein immobilizations, with expected an expected yield of 2 to 20 mg protein per column.

Supplier: Thermo Scientific

Pierce™ Protein L Plus agarose is a high-capacity Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Scientific

The GlycoLink™ Immobilisation Kit contains affinity columns and reagents to immobilise glycoproteins through oxidised sugar groups for use in affinity-purification applications or studies of protein-protein interactions. This kit is ideal for purification of antigens using covalently coupled antibodies, determining binding partners for a glycoprotein of interest and for performing large-scale affinity purifications.

Supplier: Abcam

GTP-Agarose (ab270533) comprises GTP attached to agarose beads.