Transfer Membranes

Highly sensitive transfer membranes use capillary action methods to separate DNA, RNA, or protein fragments for detection. Working well with the gels and buffers required for transfer and immobilization steps, the transfer membranes are durable enough to be subjected to multiple stripping, reprobing, hybridization cycles. The high-binding discs, sheets, and rolls come in various pore sizes and charges to fit any blotting techniques in molecular biology and genetic research.

Amersham™ Protran® Western Blotting Membranes, Nitrocellulose, Cytiva

Supplier: Cytiva

Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

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Amersham™ Hybond™ SEQ Western Blotting Membrane, 0.2 PVDF, Cytiva

Supplier: Cytiva

0.2 µm pore size for higher surface area, resulting in better binding of low molecular weight proteins. Amersham Hybond SEQ 0.2 PVDF is a 0.2 µm pore size hydrophobic membrane designed specifically for protein sequencing applications. The small pore size of this membrane minimizes ‘blow-through’ and increases protein binding over a wide range of molecular weights.

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Owl™ Immobilon™-P Transfer Membranes, PVDF, Thermo Scientific

Supplier: Thermo Fisher Scientific

Hydrophobic polyvinylidene fluoride transfer membrane has a high signal-to-noise ratio, which allows for the use of mild blocking procedures

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Whatman™ Protran® BA85 & BA83 NC Western Blotting Membranes, Nitrocellulose, Cytiva

Supplier: Cytiva

Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pre treatment steps are necessary.

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Whatman™ Nytran SuPerCharge (SPC) Blotting Membranes, Whatman products (Cytiva)

Supplier: Cytiva

Nytran® SuPerCharge (SPC) nylon membranes have a very high positive charge and a higher density of nylon per unit area. Available as circles, sheets or rolls for microplates.

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Whatman™ Nytran N Blotting Membranes, Whatman products (Cytiva)

Supplier: Cytiva

Whatman Nytran nylon membranes are available in two formats: Nytran N is moderately charged and Nytran SuPerCharge (SPC) has a very high positive charge.