Enzyme Assays

Supplier: Thermo Scientific

The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

Supplier: Thermo Scientific

Thermo Scientific Pierce APTS (3-aminopropyltriethoxysilane) is a silylation reagent for coating glass and silica surfaces to add primary amines, which can be used to crosslink and immobilize proteins and other molecules.

Supplier: Thermo Scientific

Detect more β-galactosidase activity by lysing the cells efficiently.

Supplier: Thermo Scientific

The Thermo Scientific Pierce Gaussia luciferase flash assay kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Supplier: Enzo Life Sciences

A QuantiZymeTM Assay System

Supplier: G-Biosciences

G-Biosciences' β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct.

Supplier: Enzo Life Sciences

For the measurement of Akt in partially purified, purified, or crude enzyme preparations from any species.

Supplier: Sino Biological

The substrate solution used for assaying Diacylglycerol kinases.

Supplier: Enzo Life Sciences

The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit.

Supplier: Enzo Life Sciences

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

Supplier: Thermo Scientific

Thermo Scientific Serine Hydrolase Probes are ActivX™ Fluorophosphonate (FP) and other tagged phosphonate probes to purify or assay serine hydrolase active sites using fluorescence, biotin-affinity, or mass spectrometry.

Supplier: Enzo Life Sciences

A FLUOR DE LYS® fluorescent assay system

Supplier: Enzo Life Sciences

A QUANTIZYME® Assay System Complete assay system designed to examine the specificity of inhibitors against a panel of ten matrix metalloproteinase enzymes. The assays are performed in a convenient 96-well microplate format using a sensitive fluorogenic substrate . Included are active MMP enzymes, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.

Supplier: Abcam

These Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity in vitro without lysing the cell.

Supplier: Enzo Life Sciences

It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.