Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
SensoLyte® Anti-Mouse/ Rat β-Amyloid (1-40) Quantitative ELISA (Colorimetric), AnaSpec
Supplier: Anaspec
This SensoLyte® high-sensitivity (2pg/mL) beta-Amyloid (1-40) Quantitative ELISA Kit (Mouse/Rat) provides a convenient and quantitative assay for determining mouse/rat beta-Amyloid (1-40) (Aβ40) amount in cell and tissue lysate as well as in body fluids
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SensoLyte® Rh110 Elastase Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 /520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.
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SensoLyte® Plus 520 MMP-9 Assay Kit (Fluorimetric and Enhanced Selectivity), AnaSpec Inc.
Supplier: Anaspec
The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL®520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate. Ample materials are provided to perform 96 assays in a 96-well format.
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SensoLyte® Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit, AnaSpec
Supplier: Anaspec
This kit is optimized to detect anti-human MOG (1-125) IgG in mouse serum or cerebrospinal fluid. Wells are pre-coated with recombinant human MOG (1-125) protein and pre-blocked with BSA. The amount of anti-human MOG IgG in serum or cerebrospinal fluid is quantified using ELISA. Mouse anti-human MOG IgG standard is included. Ample materials and reagents are provided to perform 96 assays in a 96-well plate format.
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AnaTag™ HiLyte™ Fluor 647 Microscale Protein Labeling Kit (Ultra Convenient), AnaSpec
Supplier: Anaspec
HiLyte™ Fluor 647 acid, SE (Ex/Em=647 nm/ 679 nm) is an excellent fluorescent labeling dye that generates the protein conjugates that are slightly red-shifted compared to those of Cy® 5 dyes, resulting in an optimal match to filters designed for Cy®5 dyes
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SensoLyte® 390 ACE2 Activity Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
ACE2 or ACEH, angiotensin-converting enzyme 2, a homolog of angiotensin-converting enzyme, is a novel zinc metallopeptidase with specificity, tissue distribution, and function distinct from those of ACE
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SensoLyte® AFC Plasmin Activity Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
Plasmin belongs to the family of serine proteases
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SensoLyte® 520 Aggrecanase-1 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Aggrecanases belong to ADAMTS (A disintegrin and metalloprotease with thrombospondin motif) family of proteases
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SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit, AnaSpec Inc.
Supplier: Anaspec
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7
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SensoLyte® 520 Cathepsin S Assay Kit Fluorimetric, AnaSpec, Inc.
Supplier: Anaspec
Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases
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SensoLyte® 520 Cathepsin K Assay Kit Fluorimetric, AnaSpec, Inc.
Supplier: Anaspec
Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption, also having a potential as a drug target in autoimmune diseases and osteoporosis
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SensoLyte® 490 MMP-13 Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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SensoLyte® Rh110 DPPIV Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
DPPIV is a widely distributed serine protease that cleaves two amino acids from small peptides containing alanine or proline in the second position of the N-terminus of the peptide
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Human TrkC Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's tropomyosin receptor kinase C (TrkC) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against TrkC.
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Human OXTR Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO's oxytocin receptor (OXTR) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against OXTR.
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Human ADRA2A Luciferase Reporter Assay Kit
Supplier: INDIGO BIOSCIENCES INC MS
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. Adrenoceptor alpha 2A (ADRA2A) reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA2A.
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SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric, AnaSpec, Inc.
Supplier: Anaspec
Alkaline phosphatase is a popular enzyme conjugated with secondary antibody for ELISA
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SensoLyte® Anti-Mouse MOG (1-125) IgG Quantitative ELISA Kit, AnaSpec
Supplier: Anaspec
This kit is optimized to detect anti-mouse MOG (1-125) IgG. Wells are pre-coated with recombinant mouse MOG (1-125) protein and pre-blocked with BSA. The amount of anti-mouse MOG IgG in serum or cerebrospinal fluid is quantified using ELISA. Ample materials and reagents are provided to perform 96 assays.
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SensoLyte® 520 Rat Renin Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme)
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SensoLyte® 520 FRET SIRT1 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins
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SensoLyte® 520 MMP-1 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease
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SensoLyte® 520 Neprilysin Activity Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Neprilysin (NEP) is a transmembrane metallopeptidase normally expressed by a variety of tissues
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SensoLyte® AMC Calpain Activity Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
The calpains are a family of intracellular Ca2+ supdependent cysteine proteases
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SensoLyte® 520 Calpain Activity Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
The calpains are a family of intracellular Ca2+ dependent cysteine proteases
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SensoLyte® ADHP Peroxidase Assay Kit (Fluorimetric), AnaSpec Inc.
Supplier: Anaspec
HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses.
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SensoLyte® FDP Alkaline Phosphatase Assay Kit Fluorimetric, AnaSpec, Inc.
Supplier: Anaspec
Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses
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SensoLyte® 520 Cathepsin L Assay Kit Fluorimetric, AnaSpec, Inc.
Supplier: Anaspec
Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases
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SensoLyte® 520 MMP-2 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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SensoLyte® 520 MMP-3 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components
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SensoLyte® 490 MMP-9 Assay Kit (Fluorimetric), AnaSpec
Supplier: Anaspec
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components