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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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GloCell™ Fixable Viability Dye Violet 450

GloCell™ Fixable Viability Dye Violet 450

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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StabilZyme® AP Conjugate stabilizer, Surmodics

Supplier: Surmodics

StabilZyme® AP and StabilZyme® Protein-Free AP stabilizers provide assay developers a complete set of tools to stabilize different AP conjugates for long term, in-solution stability.

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AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit, Anaspec

Supplier: Anaspec Inc

HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein.

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AnaTag™ HiLyte™ Fluor 555 Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec Inc

HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy™ 3 dyes, resulting in an optimal match to filters designed for CyTM dyes.

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GloCell™ Fixable Viability Dye Red 780

GloCell™ Fixable Viability Dye Red 780

Supplier: STEMCELL Technologies

Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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AnaTag™ AMCA - X Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec Inc.

Supplier: Anaspec Inc

AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group.

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AnaTag™ Biotin Protein Labeling Kit, Anaspec Inc.

Supplier: Anaspec Inc

Biotin, SE is amino-reactive biotinylating reagent for peptides and proteins; it has a better avidin-binding affinity than biotin. Biotin, SE (d-Biotin-N-hydroxysuccinimide ester) contains a five-carbon spacer arm that reduces steric hindrance associated with binding four biotinylated molecules per one avidin and results in enhanced detection sensitivity.

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AnaTag™ 5 - TAMRA Microscale Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec Inc

TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

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AnaTag™ HiLyte™ Fluor 750 Protein Labeling Kit *Ultra Convenient*, Anaspec

Supplier: Anaspec Inc

HiLyte™ Fluor 750 acid, SE is the longest-wavelength amine-reactive HiLyte™ Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647, HiLyte™ Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis.

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AnaTag™ B-PE Labeling Kit, Anaspec Inc.

Supplier: Anaspec Inc

The AnaTag™ B-PE Labeling Kit is optimized for use in the conjugation of B-Phycoerythrin (B-PE) to antibodies. B-PE, a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm.

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