Vous avez recherché : Protein Linking and Labeling Systems

Supplier: New England Biolabs (NEB)

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells

Supplier: Thermo Scientific

The DyLight™ Antibody Labeling Kits and were specifically developed for fast, efficient labeling of antibodies

Supplier: Biotium

Carbocyanine dyes with long hydrocarbon chains have low fluorescence in aqueous phase, but become highly fluorescent upon binding to lipid bilayers, making this class of dyes ideal for staining cell membranes

Supplier: Thermo Scientific

Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

Supplier: AAT BIOQUEST INC

It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: New England Biolabs (NEB)

CLIP-Surface™ 647 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells, or in vitro

Supplier: New England Biolabs (NEB)

SNAP-tag Purified Protein can be used as a positive control for in vitro labeling with various SNAP-tag fluorescent substrates

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.

Supplier: Thermo Scientific

The carbonyl-reactive Thermo Scientific™ aminoxyTMT™ (Tandem Mass Tag™) label reagents enable multiplexed characterization and quantitation of carbonyl-containing biomolecules (carbohydrates, steroids, oxidized proteins) by mass spectrometry (MS).

Supplier: New England Biolabs (NEB)

SNAP-Biotin®i s a cell-permeable substrate (BG-Biotin) based on biotin with an amidocaproyl linker

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

Supplier: G-Biosciences

The use of biotin for non-radioactive labeling of proteins and nucleic acids has now become an increasingly popular technique in life science research

Supplier: New England Biolabs (NEB)

SNAP-Cell® 430 is a blue fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

Supplier: Biotium

Biotin-20-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

Supplier: Biotium

A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.

Supplier: New England Biolabs (NEB)

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.

Supplier: New England Biolabs (NEB)

CLIP-Cell™ TMR-Star is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro

Supplier: Biotium

A lectin labeled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.

Supplier: Thermo Scientific

Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

Supplier: Thermo Scientific

Sulfo-SBED is a trifunctional crosslinking reagent containing a biotin, a sulfonated N-hydroxysuccinimide (Sulfo-NHS) active ester and a photoactivatable aryl azide

Supplier: Biotium

CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.

Supplier: Thermo Scientific

Activated peroxidase is an amine-reactive form of horseradish peroxidase (HRP) that provides coupling efficiencies of greater than 95% with antibodies and other proteins.

Supplier: New England Biolabs (NEB)

BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides

Supplier: AAT BIOQUEST INC

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: Thermo Scientific

The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.

Supplier: Thermo Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

Supplier: Thermo Scientific

DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.