Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR® Phosphate Buffered Saline (PBS), Powdered, Ultra Pure Grade

Supplier: VWR

Preparation of PBS using 10 L and 50 L Format:
Dissolve 9.88 g of PBS powder in 1 L of water to prepare a 1X PBS solution containing 137 mM sodium chloride, 2.7 mM potassium chloride and 9.8 mM phosphate buffer.
The 10 L and 50 L pack sizes contain sufficient powder to prepare 10 L and 50 L of 1X PBS, respectively.

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VWR® TRIS Buffer, 0.1 M Solution, pH 7.4

Supplier: VWR

Cell culture buffer, TRIS Buffer Solution 0.1M, pH 7.4

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VWR® MOPS Buffer Solution 10X, Biotechnology Grade

Supplier: VWR

Optimized for RNA Agarose Electrophoresis

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Options durables disponibles

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J.T.Baker®, pH Buffer Solutions

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

High quality buffer solutions ensure accurate pH measurements time after time.

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Certipur® Buffer Solution (Boric Acid/Sodium Hydroxide/Hydrogen Chloride), Traceable to SRM from NIST and PTB, MilliporeSigma

Supplier: MilliporeSigma

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Sodium hydrogen carbonate/sodium carbonate certified secondary standard reference material for pH measurement; directly traceable to primary SRM from NIST/PTB pH(S)=10.014 (25°C) (DIN 19266) Certipur®

Supplier: MilliporeSigma

Sodium hydrogen carbonate/sodium carbonate certified secondary standard reference material for pH measurement; directly traceable to primary SRM from NIST/PTB pH(S)=10.014 (25C) (DIN 19266) Certipur*