Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Pierce Y-PER™, Yeast Protein Extraction Reagent, Thermo Scientific

Supplier: Thermo Scientific

Y-PER™ reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

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Wash buffer, 0.5% Tween 20, pH 8.0, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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TRIS-buffered saline (TBS, 10X), with 1% Tween 20, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Sodium Acetate, 1 M Aqueous Solution, pH 4.5, RNAse free, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Sodium Citrate, 0.5 M Buffer Solution, pH 6.5, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Acetic Acid-Ammonium Acetate Buffer TS, Ricca Chemical Company

Supplier: Ricca Chemical

Acetic Acid-Ammonium Acetate Buffer TS, per United States Pharmacopeia (USP)

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TE buffer, pH 8.1, RNAse free, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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PIPES lysis buffer with Triton® X-100, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Tris(hydroxymethyl)aminomethane Hydrochloride, 1M stock soln, pH 8.0, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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TRIS-buffered saline (TBS, 10X) pH 7.6, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Sodium Borate, 0.5 M Buffer Solution, pH 8.5, Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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PBS 10X-Dulbecco's Phosphate Buffered Saline Solution

Supplier: FUJIFILM BIOSCIENCES INC

PBS 10X Dulbecco's Phosphate buffered saline solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.

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10X Blocking Buffer, Abcam

Supplier: Abcam

10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative

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PBS (Phosphate Buffered Saline), 10X, pH 7.4, GrowCells

Supplier: GROWCELLS

Balanced salt solution used in many biomolecular applications

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Corning® PBS (Phosphate-Buffered Saline), Corning

Supplier: Corning

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate