Next Generation Sequencing Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

NEBNext® LV Unique Dual Index Primers Set 1

Supplier: New England Biolabs (NEB)

NEBNext® LV Unique Dual Index Primers Set 1 includes 96 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ and E5hmC-seq™, with Illumina® sequencing.

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NEBNext® Ultra II FS DNA Module, New England Biolabs

Supplier: New England Biolabs (NEB)

This module is part of the NEBNext Ultra II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module, for Illumina-compatible library construction.

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NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 3)

Supplier: New England Biolabs (NEB)

NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 3) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy.

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NEXTFLEX™ 16S V1-V3 Amplicon-Sequence Kits

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX® 16S V1-V3 Amplicon-Sequence kit is designed to prepare multiplexed amplicon libraries that span the hypervariable domains one to three (V1-V3) of microbial 16S ribosomal RNA (rRNA) genes.

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NEXTFLEX™ UDI Barcodes

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX® Unique dual index barcodes are barcoded adapters that can be used with single or paired-end read sequencing library prep kits  designed for sequencing on Illumina® platforms workflows.

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NEBNext® Ultra™ II Q5® Master Mix

Supplier: New England Biolabs (NEB)

The NEBNext ultra II Q5 master mix is the most recent formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries.

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NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 4)

Supplier: New England Biolabs (NEB)

NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 4) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy.

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NEBNext® LV Unique Dual Index Primers Set 4

Supplier: New England Biolabs (NEB)

NEBNext® LV Unique Dual Index Primers Set 4 includes 96 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ and E5hmC-seq™, with Illumina® sequencing.

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NEBNext® Low-bias Small RNA Library Prep Kit

Supplier: New England Biolabs (NEB)

NEBNext® Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 to 1,000 ng) or enriched small (0.05 to 5 ng) RNA.

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NEBNEXT® End Repair Module, New England Biolabs

Supplier: New England Biolabs (NEB)

The NEBNext® End Repair Module has been optimized to repair DNA fragmented by nebulization, acoustic shearing, or nucleases

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NEBNext® Enzymatic Methyl-seq v2 Conversion Module

Supplier: New England Biolabs (NEB)

NEBNext Enzymatic Methyl-seq (EM-seq) is a high-performance enzyme-based alternative to bisulfite conversion for the identification of 5mC and 5hmC.

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Genomic Clean-up Solutions

Supplier: Beckman Coulter

CleanSEQ Sanger Dye Terminator Removal Kit is a rapid, high performance dye-terminator removal process based on the Agencourt SPRI technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performed manually or fully automated for high throughput dye-terminator removal. Compared to similar systems, the CleanSEQ system produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.

Starting sample material: DNA

Validated sample types: BigDye terminator cycle sequencing reaction products