Next Generation Sequencing Reagents

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The DOPlify® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.

Supplier: New England Biolabs (NEB)

The NEBNext^® Library Quant Kit for Ultima Genomics^® contains the reagents necessary for qPCR-based quantitation of NGS libraries prepared using the UG 100 Solaris^TM Free workflow.

Supplier: New England Biolabs (NEB)

This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module, and can be used to supplement the NEBNext Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX® 16S V1-V3 Amplicon-Sequence kit is designed to prepare multiplexed amplicon libraries that span the hypervariable domains one to three (V1-V3) of microbial 16S ribosomal RNA (rRNA) genes.

Supplier: New England Biolabs (NEB)

NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 4) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy.

Supplier: New England Biolabs (NEB)

The NEBNext ultra II Q5 master mix is the most recent formulation of Q5 DNA polymerase that has been optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries.

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX® Unique dual index barcodes are barcoded adapters that can be used with single or paired-end read sequencing library prep kits  designed for sequencing on Illumina® platforms workflows.

Supplier: New England Biolabs (NEB)

NEBNext® LV Unique Dual Index Primers Set 4 includes 96 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ and E5hmC-seq™, with Illumina® sequencing.

Supplier: New England Biolabs (NEB)

The NEBNext® End Repair Module has been optimized to repair DNA fragmented by nebulization, acoustic shearing, or nucleases

Supplier: New England Biolabs (NEB)

NEBNext® Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 to 1,000 ng) or enriched small (0.05 to 5 ng) RNA.

Supplier: New England Biolabs (NEB)

NEBNext Enzymatic Methyl-seq (EM-seq) is a high-performance enzyme-based alternative to bisulfite conversion for the identification of 5mC and 5hmC.

Supplier: Beckman Coulter

CleanSEQ Sanger Dye Terminator Removal Kit is a rapid, high performance dye-terminator removal process based on the Agencourt SPRI technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performed manually or fully automated for high throughput dye-terminator removal. Compared to similar systems, the CleanSEQ system produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.

Starting sample material: DNA

Validated sample types: BigDye terminator cycle sequencing reaction products

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX™ 16S V3-V4 Amplicon-Seq Kit is designed to prepare multiplexed amplicon libraries that span the hypervariable domains three to four (V3-V4) of microbial 16S ribosomal RNA (rRNA) genes.

Supplier: New England Biolabs (NEB)

The NEBNext ARTIC kits were developed in response to the critical need for reliable and accurate methods for sequencing SARS-CoV-2.

Supplier: New England Biolabs (NEB)

The novel NEBNext® Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries

Supplier: New England Biolabs (NEB)

NEBNext® Multiplex Oligos for Illumina are an essential piece of the NEBNext® suite of library preparation products, available in several different configurations for use with NEBNext® products or other standard Illumina-compatible library preparation protocols.