Next Generation Sequencing Reagents

Supplier: New England Biolabs (NEB)

The NEBNext Immune sequencing kit (human) enables exhaustive profiling of somatic mutations in the full-length immune gene repertoires of B cells and T cells, via the expression of complete antibody chains.

Supplier: New England Biolabs (NEB)

Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Adaptors enable high-efficiency adaptor ligation and high library yields, with minimized adaptor-dimer formation.

Supplier: New England Biolabs (NEB)

NEBNext dsDNA fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols.

Supplier: New England Biolabs (NEB)

NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 2) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy.

Supplier: New England Biolabs (NEB)

The NEBNext Globin and rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S).

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The DOPlify® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX® RiboNaut™ rRNA depletion kit (HMR) effectively removes rRNAs from mammalian total RNA while enabling labs to find additional RNA species in a sample, not only limited to intact mRNAs.

Supplier: REVVITY HEALTH SCIENCES CANADA CA

The NEXTFLEX™ small RNA-sequence kit V4 enables labs to reduce bias in their Illumina® small RNA-sequence workflows while leveraging a completely gel-free protocol, even with low input samples.

Supplier: New England Biolabs (NEB)

The NEBNext FFPE DNA repair mix contains enzymes and buffers that are optimized to repair FFPE DNA in next generation sequencing workflows.

Supplier: New England Biolabs (NEB)

NEBNext® LV Unique Dual Index Primers Set 2A includes 24 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ and E5hmC-seq™, with Illumina® sequencing.

Supplier: New England Biolabs (NEB)

The NEBNext Microbiome DNA enrichment kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment.

Supplier: New England Biolabs (NEB)

This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module, and can be used to supplement the NEBNext Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.

Supplier: New England Biolabs (NEB)

The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the 'dUTP' method for strand-specificity, with proven superiority for this application.

Supplier: New England Biolabs (NEB)

The NEBNext^® Library Quant Kit for Ultima Genomics^® contains the reagents necessary for qPCR-based quantitation of NGS libraries prepared using the UG 100 Solaris^TM Free workflow.

Supplier: REVVITY HEALTH SCIENCES CANADA CA

NEXTFLEX™ poly(A) beads 2.0 kits offer a convenient method for obtaining pure, intact mRNA upstream of RNA-sequence library prep with improved mRNA yields, low rRNA contamination and a simple protocol.

Supplier: New England Biolabs (NEB)

NEBNext® Multiplex Oligos for Illumina are an essential piece of the NEBNext® suite of library preparation products, available in several different configurations for use with NEBNext® products or other standard Illumina-compatible library preparation protocols.