Transfer Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

VWR® TRIS Glycine-SDS Buffer Powder Packs

Supplier: VWR International

VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.

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VWR® Rapid Transfer Buffer, 10X

Supplier: VWR

Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications

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VWR® TRIS Glycine Buffer

Supplier: VWR

Following electrophoresis, proteins are frequently transferred from the polyacrylamide gel onto PVDF or nitrocellulose membranes for use in immunoblotting or sequencing

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Pierce™ 10X Tris-Glycine Buffer, Thermo Scientific

Supplier: Thermo Scientific

These space-saving stock solutions are ideal for quick preparation of standard Tris-glycine (pH 8.5) transfer buffer which is used for Western blotting.

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Pierce™ 1-Step Transfer Buffer, Thermo Scientific

Supplier: Thermo Scientific

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.