Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
SideStep II Cell Lysis Analysis Kit, Agilent Technologies
Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA
Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
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Cell lysis buffer 4, (100 ml), Enzo Life Sciences
Supplier: Enzo Life Sciences
Cell lysis buffer 4, (100 ml), Enzo Life Sciences
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Lysis Buffer, FLEXLyse
Supplier: Beckman Coulter
Whole blood assays are our best approximation of physiological conditions in research flow cytometry. Density gradient leukocyte isolation or bulk lysing procedures are common to remove red blood cells, but decompose the native material prior to antigen detection. Common lysing buffers avoid this decomposition and eliminate the gradient-related preanalytical workload, but also increase sample volume beyond the single-well capacity of many plate formats, limiting throughput and automation options.
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
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RIPA buffer-2 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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NP-40 lysis buffer (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RIPA buffer with Triton® X-100 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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PhosphoSafe™ Extraction Reagent, MilliporeSigma
Supplier: MilliporeSigma
PhosphoSafe™ extraction buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.
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RBC Lysis Buffer (10X), Biolegend
Supplier: Biolegend
Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes.
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RBC Lysis Buffer, IBI Scientific
Supplier: IBI Scientific
RBC Lysis Buffer is used to lyse red blood cells in nucleic acid isolation applications.
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BipPulverizer System II, MP Biomedicals
Supplier: MP Biomedicals
BioPulverizer™ System II contains 10x2 mL tubes of each of our specialized Lysing Matrices.
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Catalog #: CA21905-026
Supplier: Kimberly-Clark