Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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Cell Lysis Buffer, Abcam

Supplier: Abcam

This product is manufactured by BioVision, an Abcam company and was previously called 1067 Cell Lysis Buffer.

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Y-Lysis Buffer, Zymo Research

Supplier: Zymo Research

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Solution 3 Neutralizing Buffer, Zymo Research

Supplier: Zymo Research

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ZR RNA Buffer, Zymo Research

Supplier: Zymo Research

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RNA Pre-Wash Buffer, Zymo Research

Supplier: Zymo Research

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SideStep II Cell Lysis Analysis Kit, Agilent Technologies

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

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Lysis Buffer, FLEXLyse

Supplier: Beckman Coulter

Whole blood assays are our best approximation of physiological conditions in research flow cytometry. Density gradient leukocyte isolation or bulk lysing procedures are common to remove red blood cells, but decompose the native material prior to antigen detection. Common lysing buffers avoid this decomposition and eliminate the gradient-related preanalytical workload, but also increase sample volume beyond the single-well capacity of many plate formats, limiting throughput and automation options.

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Cell lysis buffer 4, (100 ml), Enzo Life Sciences

Supplier: Enzo Life Sciences

Cell lysis buffer 4, (100 ml), Enzo Life Sciences

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NP-40 lysis buffer (2X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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RIPA buffer with Triton® X-100 (2X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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RIPA buffer (2X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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RIPA buffer-2 (2X), Thermo Scientific

Supplier: Thermo Scientific Chemicals

Liquid

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Extraction Buffer 5X PTR, Abcam

Supplier: Abcam

Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.