Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
NP-40 lysis buffer, low salt, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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PIPES lysis buffer with NP-40 (2X), Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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RBC lysis buffer for human, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Cell Lysis Buffer, Abcam
Supplier: Abcam
ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.
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Native lysis Buffer, Abcam
Supplier: Abcam
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
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HEPES lysis buffer with Triton|r X-100 (2X)
Supplier: Thermo Scientific Chemicals
Liquid
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Pierce™ Luciferase Cell Lysis Buffer (2X), Thermo Scientific
Supplier: Thermo Scientific
The Thermo Scientific Pierce Luciferase Cell Lysis Buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
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Pierce™ DNA Extraction Buffer, Phenol:Chloroform and Tris Buffer, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1).
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Triton® X-100 lysis buffer, pH 7.4, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Pierce™ Subcellular Protein Fractionation Kit for Tissues, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Pierce T-PER™, Tissue Protein Extraction Reagent, Thermo Scientific
Supplier: Thermo Scientific
Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples.
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Sucrose, 80%, in MNE buffer, MES + NaCl + EDTA, Thermo Scientific
Supplier: Thermo Scientific Chemicals
Liquid
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Pierce I-PER™, Insect Cell Protein Extraction Reagent, Thermo Scientific
Supplier: Thermo Scientific
I-PER™ Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculoviral-infected insect cells grown in suspension or monolayer culture.
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#: CA21905-026
: Kimberly-Clark