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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Amersham CyDye DIGE Fluors, Cytiva

Amersham CyDye DIGE Fluors, Cytiva

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

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Hydrogen Peroxide Blocking Reagent, Abcam

Supplier: ABCAM INC CA

Hydrogen peroxide blocking reagent is used to block endogenous peroxidase activity in IHC applications, enhancing signal detection in HRP / peroxidase-based systems. - Cited in over 30 publications - Endogenous peroxidase block – prevents non-specific background staining in IHC - HRP-based detection – ideal for use with HRP/peroxidase detection systems - Concentration – contains 0.3% hydrogen peroxide

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VeriBlot for IP Detection Reagent (HRP)

Supplier: ABCAM INC CA

VeriBlot for IP detection reagent (HRP) is an immunoblotting reagent that enables the trouble-free detection of immunoblotted target proteins without interference from denatured IgG. - Cited in over 80 publications - Detects target proteins without interference from IgG heavy and light chains - Recognizes non-reduced antibodies, minimizing detection of denatured IgG - Multiple species compatibility – detects IgG from bovine, goat, human, mouse, rat, rabbit, and sheep (specific isotypes detailed) - Suitable for up to 20 blots with proper dilution (1:200 in 5 ml milk)

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167Er Metal Conjugation Kit - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Labeling in < 4 hrs with 30 secs hands-on time using 167Er Metal Conjugation Kit - Lightning-Link® ab269846. 100% antibody recovery.

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ZnAF-2 DA, Enzo Life Sciences

Supplier: Enzo Life Sciences

Fluorescent probe for Zn2+

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PE/Atto 594 Conjugation Kit - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Labeling in < 4 hrs with 30 secs hands-on time using PE/Atto 594 Conjugation Kit - Lightning-Link® ab269900. 100% antibody recovery.

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Atto 633 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Labeling in < 20 mins with 30 secs hands-on time using Atto 633 Conjugation Kit (Fast) - Lightning-Link® ab269898. 100% antibody recovery.

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DyLight® 405 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Labeling in < 20 mins with 30 secs hands-on time using DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® ab201798. 100% antibody recovery.

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Atto 390 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Labeling in < 20 mins with 30 secs hands-on time using Atto 390 Conjugation Kit (Fast) - Lightning-Link® ab269895. 100% antibody recovery.

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AMCA Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

AMCA conjugation / labeling in < 20 mins with 30 secs hands-on time using AMCA Conjugation Kit (Fast) - Lightning-Link® ab195224. 100% antibody recovery.

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DyLight® 680 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

DyLight® 680 conjugation / labeling in < 20 mins with 30 secs hands-on time using DyLight® 680 Conjugation Kit (Fast) - Lightning-Link® ab201804. 100% antibody recovery.

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Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Alkaline Phosphatase conjugation / labeling in < 4 hrs with 30 secs hands-on time using Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® ab269901. 100% antibody recovery.

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Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Alkaline Phosphatase conjugation / labeling in < 4 hrs with 30 secs hands-on time using Alkaline Phosphatase Conjugation Kit (Animal Free) - Lightning-Link® ab269901. 100% antibody recovery.

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NUCLEAR-ID® Red/Green Cell Viability Reagent, Enzo Life Sciences

NUCLEAR-ID® Red/Green Cell Viability Reagent, Enzo Life Sciences

Supplier: Enzo Life Sciences

Cell viability dye.

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Latex Conjugation Kit - 400nm Red, Abcam

Supplier: ABCAM INC CA

Labeling in < 4 hrs with 30 secs hands-on time using Latex Conjugation Kit - 400nm Red ab269893. 100% antibody recovery.

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ORGANELLE-ID-RGB® Reagent I, Enzo Life Sciences

ORGANELLE-ID-RGB® Reagent I, Enzo Life Sciences

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids.

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XFD750 Conjugation Kit (BSA compatible), Abcam

Supplier: ABCAM INC CA

XFD750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using XFD750 Conjugation Kit (BSA compatible) ab312813. 100% antibody recovery.

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Amersham™ CyDye™ Value Packs (Mono-Reactive Maleimide), Cytiva

Amersham™ CyDye™ Value Packs (Mono-Reactive Maleimide), Cytiva

Supplier: Cytiva

Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labeling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Gold-Maleimide Conjugation Kit (20nm, 20 OD), Abcam

Supplier: ABCAM INC CA

20nm Gold conjugation / labeling in < 4 hrs with 30 secs hands-on time using Gold-Maleimide Conjugation Kit (20nm, 20 OD) ab269902. 100% antibody recovery.

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Phosphothreonine detection set, Enzo Life Sciences

Supplier: Enzo Life Sciences

This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites.

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iFluor® Red 750 Conjugation Kit (BSA compatible), Abcam

Supplier: ABCAM INC CA

iFluor® Red 750 conjugation / labeling in < 4 hrs with 30 secs hands-on time using iFluor® Red 750 Conjugation Kit (BSA compatible) ab312812. 100% antibody recovery.

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Amersham™ CyDye™ Mono-Reactive NHS Esters, Cytiva

Amersham™ CyDye™ Mono-Reactive NHS Esters, Cytiva

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labeling of amine groups, for example on antibodies, peptides or oligonucleotides.

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DoubleStain IHC Kit: R&Rt on human/mouse tissue (Green/HRP & AP/Red), Abcam

Supplier: ABCAM INC CA

Abcam's Doublestain IHC Kit (ab183285) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples.

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Amersham QuickStain Protein Labeling Kit, Cytiva

Amersham QuickStain Protein Labeling Kit, Cytiva

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

The Abcam PE/R-Phycoerythrin Conjugation Kit (ab102918) offers several standout features: -Rapid Conjugation: achieve PE/R-Phycoerythrin labeling in under 4 hours with just 30 seconds of hands-on time. -High Efficiency: ensures 100% antibody recovery, preserving the integrity and value of your antibodies. -Versatility: suitable for conjugating antibodies, proteins, and peptides, and the labeled antibodies can be used immediately in applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 50 publications

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Red 580 dUTP, Enzo Life Sciences

Supplier: Enzo Life Sciences

Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.

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158Gd Metal Conjugation Kit - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Labeling in < 4 hrs with 30 secs hands-on time using 158Gd Metal Conjugation Kit - Lightning-Link® ab269839. 100% antibody recovery.

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Europium-Streptavidin, Abcam

Supplier: ABCAM INC CA

Labeling in < 4 hrs with 30 secs hands-on time using Europium-Streptavidin ab270228. 100% antibody recovery.

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5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30, Abcam

Supplier: ABCAM INC CA

Oligonucleotide conjugation / labeling in < 4 hrs with 30 secs hands-on time using 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 ab270709. 100% antibody recovery.

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Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link®, Abcam

Supplier: ABCAM INC CA

Alexa Fluor® 568 conjugation / labeling in < 20 mins with 30 secs hands-on time using Alexa Fluor® 568 Conjugation Kit (Fast) - Lightning-Link® ab269821. 100% antibody recovery.

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