977 Results for: "Stemcell Technologies"

Supplier: Stemcell Technologies

Nerve growth factor (NGF)-beta is a prototypical member of the neurotrophin family and has a role in the survival and growth of neural cells, regulating cell growth, promoting differentiation into neurons, and neuron migration. The beta subtype of NGF is biologically active in comparison to the alpha-2 and gamma-2 subtypes. NGF-beta in its secreted form can bind to tyrosine kinase A (trkA) receptor with high affinity and to p75 (NTR) with low affinity (Levi and Alemà; Sofroniew et al.). NGF has been shown to possess pro-inflammatory and pro-fibrogenic properties (Micera et al.). It has also been shown that overexpression of NGF-beta promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathways (Yuan et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. PDGF-induced migration has been shown to involve MEK/ERK, EGFR, Src, and PI3K/Akt signaling pathways (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin- like fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-BB is secreted by osteoblasts to induce mesenchymal stem cell migration and angiogenesis. It has also been shown that PDGF-BB is secreted by preosteoclasts during bone modeling and remodeling to induce angiogenesis and thus proper osteogenesis (Xie et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Growth differentiation factor 5 (GDF-5) is a member of the bone morphogenetic protein (BMP) subclass of the transforming growth factor beta (TGF-β) superfamily. It binds a receptor complex comprising BMPR1B and BMPR2, which then activates the SMAD signaling pathway (Carreira et al.; Nishitoh et al.; Osório et al.). GDF-5 enhances chondrogenic differentiation of mesenchymal stem cells, skeletogenesis, and dendrite growth during development (Coleman et al.; Francis-West et al.). Studies in rat demonstrated that GDF-5 regulates patterning, neurogenesis, astrogliogenesis, and neuronal specification (Gajavelli et al.; Krieglstein et al.; O’Keeffe et al.).

Supplier: Stemcell Technologies

Interleukin 21 (IL-21) is a pleiotropic cytokine that is composed of four α-helical bundles and primarily produced by natural killer T (NKT) cells, T follicular helper (Tfh) cells, and Th17 cells (Spolski and Leonard 2008). IL-21 signals via heterodimers of the IL-21 receptor (IL-21R) and the IL2RG encoded common cytokine receptor γ-chain (Parrish-Novak et al.; Ozaki K et al. 2000), and utilizes the JAK/STAT, MAPK, and PI3K pathways (Spolski and Leonard 2014). IL-21 has been shown to have a critical role in regulating immunoglobulin production and differentiation of the pro-inflammatory Th17 population of cells (Ozaki et al. 2002; Nurieva et al.). Additionally, IL-21 specifically sustains CD8+ T cell effector activity and provides a mechanism of CD4+ T cell help during chronic viral infection (Elsaesser et al.). IL-21 signaling was also found critical for the development of type 1 diabetes in NOD mice (Sutherland et al.) and control of T cell autoimmunity by regulatory B cells (Yoshizaki et al.).

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human, monkey CD137 (ILA).

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human, monkey CD137 (ILA).

Supplier: Stemcell Technologies

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b.

Supplier: Stemcell Technologies

Hamster (Armenian) monoclonal antibody against mouse T cell receptor beta, PE-conjugated.

Supplier: Stemcell Technologies

R-Spondin-3 is a member of thrombospondin type 1 repeat (TSR-1) superfamily that is involved in the canonical Wnt/β-catenin signaling pathway (de Lau et al.). R-spondin proteins are characterized by two furin-like repeats at the amino terminus and thrombospondin domain located near the carboxyl terminus (de Lau et al.). R-spondin-3 expression is associated with ovarian cancer (Gu et al.), prostate cancer (Mesci et al.), and differentiation of intestinal epithelial cells in diabetes mellitus (Shan et al.). In a transgenic mice model, the expression of R-Spondin-3 induces the expansion of Lgr5+ stem cells, Paneth cells, and Lgr4+ cells, promoting the intestinal stem cell compartment (Hilkens et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain.

Supplier: Stemcell Technologies

Mouse monoclonal antibody against mouse CD45.2, APC-conjugated.

Supplier: Stemcell Technologies

Rat monoclonal antibody against mouse CD127, PE-conjugated.

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human, rhesus CD80 (B7-1), APC-conjugated.

Supplier: Stemcell Technologies

Rat monoclonal antibody against mouse, hamster CD4, APC-conjugated.

Supplier: Stemcell Technologies

Defined supplement for single-cell cloning of human ES and iPS cells.

Supplier: Stemcell Technologies

Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mm HEPES buffer.

Supplier: Stemcell Technologies

Defined cell culture medium for expansion of reset naïve human embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Supplier: Stemcell Technologies

Cell dissociation reagent.

Supplier: Stemcell Technologies

Serum- and BPE-free medium for expansion of primary human airway epithelial cells.

Supplier: Stemcell Technologies

Medium for detection of CFU-F and expansion of human mesenchymal stem cells.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human pan-granulocytes

Supplier: Stemcell Technologies

Immunodensity isolation of untouched monocytes.

Supplier: Stemcell Technologies

Serum-free neurophysiological basal medium for improved neuronal function.

Supplier: Stemcell Technologies

Serum-free culture supplement for expansion of human erythroid cells.

Supplier: Stemcell Technologies

Complete kit including collagen, media, staining kit and chamber slides for human CFU-Mk assays.

Supplier: Stemcell Technologies

Defined, serum-free, xeno-free medium for pluripotent stem cells.

Supplier: Stemcell Technologies

Animal component-free collagenase for the digestion of native collagen fibrils.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human, mouse CD105 (endoglin).

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human, rhesus, cynomolgus CD81, unconjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgG2a, kappa isotype control antibody, unconjugated.

Supplier: Stemcell Technologies

For detection and measurement of human interleukin 4.