977 Results for: "Stemcell Technologies"

Supplier: STEMCELL Technologies

Medium without cytokines.

Supplier: STEMCELL Technologies

Rat (WI) monoclonal IgG2b antibody against mouse TER119.

Supplier: STEMCELL Technologies

Goat polyclonal antibody against mouse IgG (H+L).

Supplier: STEMCELL Technologies

For detection and measurement of human interleukin 5.

Supplier: STEMCELL Technologies

Interleukin 13 (IL-13) is a type I cytokine, and signals through type I cytokine receptors to activate JAK/STAT and IRS-1/IRS-2 pathways. IL-13 is produced by T cells and innate lymphoid cells (Pulendran and Artis) and it inhibits production of pro-inflammatory cytokines, prostaglandins, and reactive oxygen and nitrogen species by monocytes and macrophages (Hershey). Unlike human B cells, IL-13 has no effect on mouse B cell development and function. IL-13 promotes eosinophil survival, activation, and recruitment, and also activates mast cells (Hershey). IL-13 regulates gastrointestinal parasite expulsion, airway hyperresponsiveness, allergic inflammation, tissue eosinophilia, goblet cell hyperplasia, intracellular parasitism, tissue remodeling, tumor cell growth, and fibrosis. IL-13 induces hypercontractility of smooth muscles by directly acting on the muscle cells as well as the enteric nerves (Wynn).

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human IFN-gamma, unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee, gibbon TRA-2-49 (tissue non-specific alkaline phosphatase), unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse CD8a, unconjugated.

Supplier: STEMCELL Technologies

For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages.

Supplier: STEMCELL Technologies

Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann and Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation, and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).

Supplier: STEMCELL Technologies

Methylcellulose-based medium with EPO for human cells.

Supplier: STEMCELL Technologies

Integrated workflow containing tools to isolate and expand CD34+ progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells.

Supplier: STEMCELL Technologies

Use autotaxin (ENPP2) to catalyze the production of lysophosphatidic acid (LPA), a potent mitogen that can evoke growth factor-like responses (Moolenaar and Corven), from lysophospholipids in extracellular fluids. Autotaxin is a secreted glycoprotein that belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (ENPP) family, containing two N-terminal somatomedin B (SMB)-like domains, a central phosphodiesterase (PDE) domain with an active catalytic site, and a C-terminal nuclease-like (NUC) domain (Nishimasu et al.). Dysregulation of autotaxin and LPA receptors is implicated in cancer (Tigyi et al.), fibrosis (Ninou et al.), neurological disorders (Roy et al.), and other inflammation-associated conditions. Both Autotaxin and LPA are overexpressed in many cancers and can promote cell proliferation, migration, and resistance to apoptotic death (Tigyi et al.). Autotaxin was also found to catalyze the production of cyclic phosphatidic acid (CPA), an analog of LPA, which has anti-mitogenic and inhibitory effects on tumor cell invasion and metastasis (Fujiwara). This protein contains a His-residue tag at the amino end of the polypeptide chain. For consistency and reproducibility across your applications, Autotaxin from STEMCELL comes lyophilized with ≥ 85% purity, and endotoxin levels are verified to be ≤1.0 EU/μg protein.

Supplier: STEMCELL Technologies

Reliably isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™, a cost-effective alternative to Ficoll-Paque™.

Supplier: STEMCELL Technologies

Cas9 nuclease (4 μg/μl) for the generation of double-strand breaks in CRISPR-Cas9 genome editing.

Supplier: STEMCELL Technologies

Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin supports maintenance of undifferentiated human embryonic stem cells in vitro, and can be used to prevent spontaneous differentiation in the short term (Chaturvedi et al.). Noggin is essential for development of structures derived from ectoderm embryonic somite, skeletal patterning, and neurogenesis in vivo. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al.), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al.).

Supplier: STEMCELL Technologies

Stimulating T cells with EBV (HLA Class I Control) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (HLA Class I Control) Peptide Pool is a lyophilized mixture of 26 peptides from Epstein-Barr virus (EBV), and consists of defined HLA class I-restricted T cell epitopes. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development. For consistency and reproducibility across your applications, CMV peptide pool from STEMCELL has a purity ≥95%.

Supplier: STEMCELL Technologies

Complete kit for optimization of genome editing in human cells.

Supplier: STEMCELL Technologies

Immunodensity isolation of untouched NK cells.

Supplier: STEMCELL Technologies

Serum-free culture supplement for expansion of human hematopoietic cells.

Supplier: STEMCELL Technologies

cGMP, feeder-free maintenance medium for human ES and iPS cells.

Supplier: STEMCELL Technologies

Interferon gamma-inducible protein (IP) 10 or CXCL10 is a member of the CXC chemokine family. It binds CXCR3 activating ERK1/2, p38/MAPK, JNK, and PI3-kinase/AKT signaling pathways, inducing intracellular calcium influx, DNA synthesis, cell proliferation, and chemotaxis. IP-10 regulates innate and adaptive immune responses by affecting the function of activated T cells, natural killer cells, inflammatory dendritic cells, macrophages, and B cells. IP-10 is produced by leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, fibroblasts, and keratinocytes in response to IFN-gamma. IP-10 has been implicated in a wide range of diseases, including infectious diseases and chronic inflammatory and autoimmune diseases, as well as in tumor formation (Liu et al.).

Supplier: STEMCELL Technologies

Dickkopf-related protein 1 (DKK-1) is a member of the Dickkopf family and is a secreted protein that inhibits the canonical WNT pathway by competitive binding to low-density lipoprotein receptors (LRP)-5 and 6 with high affinity, thereby decreasing β-catenin protein stability (Niehrs). DKK-1 regulates embryonic development and contains two conserved cysteine-rich domains separated by a linker region and an N-terminal signal peptide (Krupnik et al.; Lieven et al.). A family of human DKK-related genes composed of DKK-1, DKK-2, DKK-3, and DKK-4 have been characterized together with a unique DKK-3 related protein termed Soggy (Krupnik et al.). DKK-1 has been shown to support the generation of myeloid-derived suppressor cells (MDSCs) and thus is a negative regulator of antitumor immune responses (D’Amico et al.). DKK-1 from thrombocytes is an important regulator of leukocyte infiltration and induces Th2 cell polarization and potentiates Th2 cell cytokine expression (Chae et al.). DKK-1 has also been shown to drive cardiac and retinal differentiation from induced pluripotent stem (iPS) cells (Lian et al.). Protein contains a His-residue tag at the carboxyl end of the polypeptide chain.

Supplier: STEMCELL Technologies

Density gradient medium.

Supplier: STEMCELL Technologies

cGMP, human T cell activation and expansion reagent.

Supplier: STEMCELL Technologies

The EasySep™ 'The Big Easy' magnet is designed for cell separation procedures using EasySep™ reagents when processing a large number of cells, or when using RoboSep™.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of mouse naïve CD4+ T cells.

Supplier: STEMCELL Technologies

The EasySep™ Easy 50 magnet is designed for cell separation procedures using selected EasySep™ reagents when processing a large number of cells (up to 4×10⁹ cells).

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human plasmacytoid dendritic cells (pDC)

Supplier: STEMCELL Technologies

Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor (Gaffen and Liu). It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival (Ma et al.). The majority of IL-2 is secreted by activated CD4+ and CD8+ T cells, although B cells and dendritic cells were found to produce IL-2 in small amounts. IL-2 downregulates immune responses to prevent autoimmunity during thymic development, influences the development of CD4+CD25+ regulatory T cells, and affects development of follicular helper T cells. IL-2 also controls inflammation by inhibiting Th17 differentiation (Banchereau et al.). High IL-2 levels in serum are associated with progression of scleroderma, rheumatoid arthritis, and gastric and non-small cell lung cancer, though no known disease can be directly attributed to the lack or excess of IL-2 (Gaffen and Liu).