Supplier: Stemcell Technologies

Serum-free neural supplement without insulin.

Supplier: Stemcell Technologies

0.25% porcine trypsin in citrate saline solution for the detachment of adherent cells.

Supplier: Stemcell Technologies

Xeno-free and serum-free medium for culture and expansion of human hematopoietic cells.

Supplier: Stemcell Technologies

Immunomagnetic enrichment of untouched human hematopoietic progenitor cells.

Supplier: Stemcell Technologies

Mouse monoclonal IgM antibody against human, mouse, rat SSEA-1 (CD15), unconjugated.

Supplier: Stemcell Technologies

Rabbit polyclonal IgG antibody against mouse Ym1, unconjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human SSEA-5, unconjugated.

Supplier: Stemcell Technologies

Apoptosis staining and detection kit - 100 tests.

Supplier: Stemcell Technologies

Growth differentiation factor 11 (GDF-11) is a member of transforming growth factor beta (TGF-β) family. It binds to the TGF-β receptors ALK4, ALK5, and ALK7 and activates the SMAD signaling pathway (Ho et al.). GDF-11 regulates the development of the olfactory system, retina, and pancreas, as well as anterior/posterior patterning of the axial skeleton (Lee and Lee). GDF-11 is an endocrine factor expressed in skeletal muscle, brain, and dental pulp (Kondás et al.). Studies in mice showed that GDF-11 regulates muscle and cardiac aging, and stimulates neurogenesis by remodeling blood vessels (Katsimpardi et al.; Loffredo et al.; Sinha et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Use Interferon beta (IFN-β) to modulate the activity of genes that control dendritic cell activation, T cell survival, NK cell activation, chemokine expression, lymph node retention, and antiproliferative and antiviral effects (Dunn et al. Nat Rev Immunol, 2006). IFN-β binds to a receptor complex composed of IFNAR1 and IFNAR2, and initiates signal transduction via the JAK/STAT pathway. It is predominantly produced by fibroblasts, with smaller amounts from plasmocytoid dendritic cells. Macrophages and endothelial cells secrete IFN-β in response to viral infection (Reder and Feng. Front Immunol, 2013). IFN-β suppresses Th17 cells by affecting expression of IL-4, IL-10, and IL-27, and is a first-line treatment for multiple sclerosis. IFN-β was also shown to expand regulatory T cells and limit T cell trafficking to the central nervous system (Inoue and Shinohara. Immunology, 2013). Of the two IFN-β variants (IFN-β1 and IFN-β3), this product is the IFN-β1 form.

Supplier: Stemcell Technologies

Serum-free supplement for the derivation and expansion of human skeletal muscle progenitor cells (myoblasts).

Supplier: Stemcell Technologies

Myostatin is a member of transforming growth factor beta (TGF-β) superfamily that signals by binding to activin type II receptor, resulting in the recruitment of either ALK3 or ALK4 coreceptor, and activation of SMAD2/3 (Kondás et al.). It is released by myocytes and inhibits muscle growth and ability to regenerate (Lee and Lee). Myostatin appears to affect the lipid catabolic metabolism of adipocytes and inhibits preadipocyte differentiation, as demonstrated in the 3T3-L1 cell line (Li et al.).

Supplier: Stemcell Technologies

Add this high-quality human recombinant transforming growth factor beta 1 (TGF-β1) to your cell culture workflow. Its verified endotoxin level of ≤0.2 EU/μg, measured using the LAL method, allows confidence for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.

Supplier: Stemcell Technologies

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge-stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin- like fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). It has been shown that PDGF-AB together with 5-Azacytidine (Catalog #72012), induces the conversion of mature bone and fat cells into tissue-regenerative multipotent stem cells (Chandrakanthan et al.).

Supplier: Stemcell Technologies

Stimulating T cells with EBV (LMP1) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. EBV (LMP1) Peptide Pool is a lyophilized mixture of 94 peptides from latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV; strain B95-8), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 386 on LMP1. LMP1 is essential for EBV-mediated growth transformation of B cells (Kaye et al.) and has been shown to downregulate toll-like receptor 9 (TLR9) expression in infected cells, a mechanism the virus may use to escape host innate immune recognition (Fathallah et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: Stemcell Technologies

SARS-CoV-2 Recombinant Nucleocapsid Protein, aa1-419 is expressed in E. coli and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Nucleocapsid Protein is transcribed from the viral “N” gene and is the protein that interacts with RNA to form the nucleocapsid. The protein is a homo-oligomer, and both the monomer and the oligomer can interact with RNA. This protein also interacts with the membrane protein (protein M) after infection of the host cell during packaging of the positive-strand viral genome RNA into the ribonucleocapsid during virion assembly. At the amino terminus, SARS-CoV-2 Recombinant Nucleocapsid Protein contains a thrombin site, a T7 tag, and a polyhistidine tag.

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human TNF-alpha, PF488P-conjugated.

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human perforin, unconjugated.

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human IFN-gamma, FITC-conjugated.

Supplier: Stemcell Technologies

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b.

Supplier: Stemcell Technologies

FRET-based peptide substrate for detection of protease activity of coronaviruses - 100 tests.

Supplier: Stemcell Technologies

Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor (Gaffen and Liu). It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival (Ma et al.). The majority of IL-2 is secreted by activated CD4+ and CD8+ T cells, although B cells and dendritic cells were found to produce IL-2 in small amounts. IL-2 downregulates immune responses to prevent autoimmunity during thymic development, influences the development of CD4+CD25+ regulatory T cells, and affects development of follicular helper T cells. IL-2 also controls inflammation by inhibiting Th17 differentiation (Banchereau et al.). High IL-2 levels in serum are associated with progression of scleroderma, rheumatoid arthritis, and gastric and non-small cell lung cancer, though no known disease can be directly attributed to the lack or excess of IL-2 (Gaffen and Liu).

Supplier: Stemcell Technologies

Rat monoclonal antibody against mouse CD3, unconjugated.

Supplier: Stemcell Technologies

Immunomagnetic isolation of neutrophils directly from whole blood

Supplier: Stemcell Technologies

Cell detachment solution.

Supplier: Stemcell Technologies

Methylcellulose-based medium with agar-LCM and EPO for human cells.

Supplier: Stemcell Technologies

Semi-solid methylcellulose-based medium for selection and cloning of suspension-adapted and adherent cells (serum-containing).

Supplier: Stemcell Technologies

Animal component-free collagenase for the digestion of native collagen fibrils.

Supplier: Stemcell Technologies

Immunodensity pre-enrichment of untouched progenitor cells from cord blood.

Supplier: Stemcell Technologies

Mouse monoclonal IgG2a, antibody against human CD3, FITC-conjugated.