977 Results for: "Stemcell Technologies"

Supplier: Stemcell Technologies

Interleukin 33 (IL-33) is a pro-inflammatory cytokine from the IL-1 family. It binds to ST2 receptor and activates NF-κB and MAPK pathways. IL-33 is expressed by epithelial cells, smooth muscle cells, and fibroblasts in various tissues and organs, as well as resting basophils, mast cells, eosinophils, natural helper cells, group 2 innate lymphoid cells, dendritic cells, and activated macrophages (Schmitz et al.; Yasuda et al.). It contributes to allergic inflammation by stimulating production of the cytokines IL-4, IL-5, and IL-13, and stimulates host defense against microbial and viral infections (Liew; Yasuda et al.). In the central nervous system, IL-33 is produced by endothelial cells and astrocytes. It induces proliferation of microglia and mediates production of pro-inflammatory cytokines (Yasuoka et al.).

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Interleukin 6 (IL-6) is a pleiotropic growth factor with the wide range of biological activities in immune regulation, hematopoiesis, and oncogenesis. IL-6 is produced by a variety of cell types including T cells, B cells, monocytes and macrophages, fibroblasts, hepatocytes, vascular endothelial cells, and various tumor cell lines. On its own or in combination with other factors such as IL-2 and interferon-γ, IL-6 stimulates the proliferation of B cells, T cells, and hybridoma cells (Hirano et al.; Mihara et al.; Tanaka et al). In combination with cytokines such as IL-3, GM-CSF and SCF, IL-6 has been shown to promote hematopoietic progenitor cell proliferation and differentiation in vitro. IL-6 signals through a cell surface type I cytokine receptor complex consisting of the ligand-binding IL-6α (CD126) and the signal-transducing gp130 subunits. The binding of IL-6 to its receptor system includes activation of JAK/STAT signaling pathway (Mihara et al.; Peters et al.; Tanaka et al.).

Supplier: Stemcell Technologies

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitors to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux andamp; Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam andamp; Ghosh). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam andamp; Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human, rhesus, cynomolgus CD81, unconjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgG2a, kappa isotype control antibody, unconjugated.

Supplier: Stemcell Technologies

For detection and measurement of human interleukin 4.

Supplier: Stemcell Technologies

For expansion and differentiation of hPSCs to NK cells.

Supplier: Stemcell Technologies

Rat monoclonal IgM, kappa isotype control antibody, unconjugated.

Supplier: Stemcell Technologies

For detection and measurement of human interleukin 12 (p70) subunit.

Supplier: Stemcell Technologies

Mouse monoclonal IgM antibody against human, chimpanzee CD66b, unconjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1, antibody against human, chimpanzee CD4, FITC-conjugated.

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Immunomagnetic positive selection of human CD138+ cells (e.g. normal or malignant plasma cells for multiple myeloma research).

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Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids.

Supplier: Stemcell Technologies

(C3H x BALB/c) F1 hybrid monoclonal IgG2a antibody against mouse NK1.1 (CD161).

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Amine-labeling fluorescent dye for live/dead staining of mammalian cells - 100 tests.

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Conditional reprogramming medium for epithelial cell expansion.

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Immunomagnetic positive selection kit.

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Immunomagnetic enrichment of untouched human ILC2 cells.

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Immunodensity isolation of untouched circulating tumor cells.

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Mouse monoclonal IgG1 antibody against human, mouse CD105 (endoglin).

Supplier: Stemcell Technologies

Mediate calcium phosphate clearance and prevent ectopic calcification with fetuin A, a plasma glycoprotein that forms soluble complexes with calcium and phosphate (Heiss et al.; Price and Lin). Belonging to the cystatin superfamily of cysteine protease inhibitors (Brown and Dziegielewska), fetuin A has also been shown to play a role in lipid transport, acting as a carrier (Kumbla et al.). In cell-based assays, it has been suggested that fetuin A protects against lethal systemic infection through the inhibition of high mobility group box 1 (HMGB1) protein accumulation and release (Li et al.). Fetuin A acts as a natural antagonist against specific TGF-β and BMP signaling proteins, blocking osteogenic differentiation of rat bone marrow cells (Demetriou et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, fetuin A from STEMCELL comes lyophilized with ≥94% purity, and endotoxin levels are verified to be ≤1.0 EU/μg protein.

Supplier: Stemcell Technologies

R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat and Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R­-Spondin-1 activates β­-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein 6 (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK­1 for binding to the WNT co­receptors, Kremen and LRP­6, which reduces DKK­1-­mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells (Cruciat and Niehrs; de Lau et al.; Genthe and Clements; Liu et al.).

Supplier: Stemcell Technologies

Use omentin for the maintenance of insulin sensitivity and metabolism, decreasing levels of this adipokine have been associated with obesity, insulin resistance, and diabetes (Tan et al.). Studies have suggested that omentin may have protective effects against atherosclerosis, arterial calcification, and myocardial injury through AMP-activated protein kinase (AMPK) and Akt-dependent mechanisms (Xu et al., Kataoka et al.). Through regulation of Sirt1-dependent p53 deacetylation, omentin can inhibit proliferation and induce apoptosis in hepatocellular carcinoma cells (Zhang and Zhou). Omentin is mainly produced by visceral adipose tissue, but is also expressed in the small intestine, colon, mesothelial cells, vascular cells, and plasma (Watanabe et al). Protein sequence analysis has shown that omentin comprises 313 amino acids, with a secretory signal sequence and fibrinogen-related domain (Yang et al.). For consistency and reproducibility across your applications, Omentin from STEMCELL comes lyophilized with ≥90% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤5,0 EU/μg protein.

Supplier: Stemcell Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).

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Isolate or deplete highly purified HLA-DR+ cells from human PBMCs or leukapheresis samples by immunomagnetic selection.

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Commonly known as vaspin, Serpin A12 is an adipokine that is thought to perform insulin-sensitizing functions, such as the regulation of kallikrein 7 (KLK7), an insulin-inhibiting protease (Heiker et al.). The Serpin A12 glycoprotein belongs to the serine protease inhibitor family (serpin), and is mainly associated with obesity, insulin resistance, and glucose metabolism, but may also protect against high fat-induced bone loss (Wang et al.). Serpins share a highly conserved core structure with multiple α-helices, β-strands, and a reactive site loop (RCL) to interact with the target protease (Huntington). It is expressed predominantly in visceral adipose tissue and at significantly higher amounts in obese individuals (Kurowska et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, Serpin A12 from STEMCELL comes lyophilized with ≥ 94% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1.0 EU/μg protein.

Supplier: Stemcell Technologies

Stimulating T cells with Candida (MP65) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. Candida peptide pool is a lyophilized mixture of 92 peptides from the mannoprotein MP65 of Candida albicans (yeast), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 379 on MP65. MP65 contributes to cell wall integrity and epithelia adherence (Sandini et al.), and is a potent T cell immunogen (Gomez et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

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For culture and expansion of human hematopoietic cells.

Supplier: Stemcell Technologies

Serum-free and animal component-free supplement for expansion of NK cells.

Supplier: Stemcell Technologies

Fluorescent dye conjugates for staining cell membranes of gram-positive bacteria, mammalian cells, and yeast bud scars - 1000 µg.