You searched for: Reverse Transcription Reagents

Supplier: Quantabio

UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems that do not require a passive reference dye. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. qScript XLT One-Step RT-qPCR ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Enzo Life Sciences

Fluorescein-12-UTP is ideal for in vitro transcription reactions catalyzed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridization to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridization procedures.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

The universal reverse primer serves as the downstream primer in the qPCR reaction by annealing to a universal sequence tag that is added to the cDNA during the reverse transcription protocol.

Supplier: Cytiva

Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.

Supplier: New England Biolabs (NEB)

Bst 3.0 DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity

Supplier: AAT BIOQUEST INC

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling or PCR.

Supplier: New England Biolabs (NEB)

Most eukaryotic mRNAs require a 7-methyl guanosine (m7G) cap structure at the 5´ end and a Poly(A) tail at the 3´ end to be efficiently translated.

Supplier: Zymo Research

The Quick-DNA/RNA MiniPrep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality genomic DNA and total RNA that is ready for any downstream application including reverse transcription, microarray, sequencing, etc.

Supplier: Teknova

First strand synthesis buffer designed for use in reverse transcription.

Supplier: Zymo Research

The RNA Clean & Concentrator™ (RCC™) kits facilitate the efficient removal of RNA polymerases, ligases, and RNA modifying enzymes.

Supplier: Zymo Research

ZymoScript RT PreMix is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis.

Supplier: New England Biolabs (NEB)

The Monarch spin PCR and DNA cleanup kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription.

Supplier: AGILENT TECHNOLOGIES (GENOMICS) CA

AccuScript High-Fidelity Reverse Transcriptase delivers the highest RT accuracy currently available.

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

Supplier: Quantabio

SuperMix provides a sensitive and easy-to-use solution for two-step qRT-PCR

Supplier: Quantabio

The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR.

Supplier: Quantabio

qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR

Supplier: Quantabio

Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Supplier: Lucigen

Lucigen’s NxGen™ M-MulV Reverse Transcriptase is outstanding in cDNA synthesis.

Supplier: Quantabio

These easy-to-use and highly efficient kits are designed for the synthesis of first-strand cDNA using your choice of cDNA priming method

Supplier: New England Biolabs (NEB)

Most eukaryotic mRNAs require a 7-methyl guanosine (m7G) cap structure at the 5´ end and a poly(A) tail at the 3´ end to be efficiently translated.

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

Supplier: Tonbo Biosciences

The FastSCRIPT cDNA synthesis kit contains everything to generate unbiased, efficient, sensitive high-quality cDNA for downstream experiments

Supplier: New England Biolabs (NEB)

ProtoScript® First Strand cDNA Synthesis Kit features two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix

Supplier: MilliporeSigma

The One Step RT-PCR Master Mix Kit allows for rapid analysis of gene expression from tissues and cells

Supplier: AAT BIOQUEST INC

This iFluor 488-dUTP conjugate is used as a green fluorescence color with SpectrumGreen™ filter set (SpectrumGreen™ is the trademark of Vysis).

Supplier: AAT BIOQUEST INC

This iFluor® 555-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™ Alexa Fluor 555-dUTP.

Supplier: Avantor

The cDNA-PCR Sequencing Kits enable the identification and quantification of full-length transcripts with highest output using nanopore sequencing.

Supplier: AAT BIOQUEST INC

This iFluor® 488-PEG12-dUTP conjugate has the same spectral properties to ChromaTide™ Alexa Fluor 488-dUTP.