93 Results for: "QUALITY BIOLOGICAL, INC."

Supplier: QUALITY BIOLOGICAL, INC.

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.

Supplier: QUALITY BIOLOGICAL, INC.

DNA Gel Loading Solutio, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer.

Supplier: QUALITY BIOLOGICAL, INC.

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.

Supplier: QUALITY BIOLOGICAL, INC.

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989)

Supplier: QUALITY BIOLOGICAL, INC.

Hanks' Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbohydrate as an energy source.

Supplier: QUALITY BIOLOGICAL, INC.

Sodium acetate is a widely used reagent in molecular biology applications. It is used as a buffer in conjunction with acetic acid, in the buffering range of pH 3.6 – 5.6.

Supplier: QUALITY BIOLOGICAL, INC.

QBI’s Water, Cell Culture, WFI Quality is high quality water for use as a solvent in the preparation of cell culture media and biochemical reagents.

This water is extensively purified by reverse osmosis, subsequently deionized and passed through a filter membrane to remove particles and tested to meet the specifications found in the USP (United States Pharmacopeia) monograph for Water for Injection (WFI) for conductivity, endotoxin,and TOC. It is additionally tested for sterility, mycoplasma, and cell culture performance.

This water is tested against USP specifications, but is not intended for injection. It is only intended for research or further manufacturing use, not for diagnostic or therapeutic use.

Supplier: QUALITY BIOLOGICAL, INC.

Terrific Broth was developed by Tartoff and Hobbs to increase yields in plasmid bearing E. coli. Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield.

Supplier: QUALITY BIOLOGICAL, INC.

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E.

Supplier: QUALITY BIOLOGICAL, INC.

Penicillin-Streptomycin acts in two ways. Penicillin inhibits bacterial cell wall synthesis. While, streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Supplier: QUALITY BIOLOGICAL, INC.

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.

Supplier: QUALITY BIOLOGICAL, INC.

TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.

Supplier: QUALITY BIOLOGICAL, INC.

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Supplier: QUALITY BIOLOGICAL, INC.

Sodium Dodecyl Sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods; this SDS is lauryl-grade.

Supplier: QUALITY BIOLOGICAL, INC.

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes.

Supplier: QUALITY BIOLOGICAL, INC.

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Supplier: QUALITY BIOLOGICAL, INC.

MOPS buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane.

Supplier: QUALITY BIOLOGICAL, INC.

Fischer’s medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD), for serial propagation of cells from leukemic mice. Fischer’s medium supports clonal growth of cells – particularly lymphoblasts – from primary explants or cells already in culture.

Supplier: QUALITY BIOLOGICAL, INC.

YPD is a media variant derived from YPAD. YPAD consists of nutrient base (yeast extract and peptone), adenine supplement and a carbon source. YPD’s formulation is the same but without the additional adenine.

YPD 1/2 Plates are useful for subculturing of untransformed yeast, yeast two-hybrid screening, yeast one-hybrid screening, enrichment and isolation of yeast; ade2 yeast will turn red on YPD.

Supplier: QUALITY BIOLOGICAL, INC.

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization

Supplier: QUALITY BIOLOGICAL, INC.

Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and elicits miscoding.

Supplier: QUALITY BIOLOGICAL, INC.

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water.

Supplier: QUALITY BIOLOGICAL, INC.

Non Fat Dry Milk

Supplier: QUALITY BIOLOGICAL, INC.

USDA FBS is used as a supplement in classical cell culture media. USDA FBS both nourishes and protects the cells in a way that almost nothing else has been found to do.

Supplier: QUALITY BIOLOGICAL, INC.

Cryopreservation Medium is a ready-to-use medium for the preservation of cells as seed stock or for future investigative study. Cryopreservation allows you to preserve and protect your cell line stock(s) from over culturing that can lead to unwanted changes within cellular properties or lead to senescence.

Supplier: QUALITY BIOLOGICAL, INC.

Compared to other basal media, Ham’s F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Ham’s F-12 contains no proteins or growth factors, and uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH.

Supplier: QUALITY BIOLOGICAL, INC.

YPG Agar is recommended for the growth of Saccharomyces cerevisiae for molecular biology purpose.

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INWATER CELL CULTURE GRADE 10LIN

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INFBS, RESEARCH GRADE USDAIN

Supplier: QUALITY BIOLOGICAL, INC.

INFBS, HI, RESEARCH GRADE USDAIN