93 Results for: "QUALITY BIOLOGICAL, INC."

Supplier: QUALITY BIOLOGICAL, INC.

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E.

Supplier: QUALITY BIOLOGICAL, INC.

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production.

Supplier: QUALITY BIOLOGICAL, INC.

DNA Gel Loading Solutio, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer.

Supplier: QUALITY BIOLOGICAL, INC.

EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators.

Supplier: QUALITY BIOLOGICAL, INC.

RNA Gel Loading Solution, 10X is prepared according to Sambrook, Fritsch & Maniatis (1989)

Supplier: QUALITY BIOLOGICAL, INC.

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.

Supplier: QUALITY BIOLOGICAL, INC.

Sodium acetate is a widely used reagent in molecular biology applications. It is used as a buffer in conjunction with acetic acid, in the buffering range of pH 3.6 – 5.6.

Supplier: QUALITY BIOLOGICAL, INC.

YPD is a media variant derived from YPAD. YPAD consists of nutrient base (yeast extract and peptone), adenine supplement and a carbon source. YPD’s formulation is the same but without the additional adenine.

YPD 1/2 Plates are useful for subculturing of untransformed yeast, yeast two-hybrid screening, yeast one-hybrid screening, enrichment and isolation of yeast; ade2 yeast will turn red on YPD.

Supplier: QUALITY BIOLOGICAL, INC.

TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.

Supplier: QUALITY BIOLOGICAL, INC.

SSC 20X buffer is a ready-to-use standard reagent in Southern and Northern hybridization procedures used to control stringency for the washing steps after hybridization

Supplier: QUALITY BIOLOGICAL, INC.

Tris-acetate provides electrical conductivity and maintains pH. EDTA inhibits metal dependent nucleases by chelating the divalent cations (Ca2 , Mg2 ), thus protecting the DNA from nucleases during the run.

Supplier: QUALITY BIOLOGICAL, INC.

Sodium Dodecyl Sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods; this SDS is lauryl-grade.

Supplier: QUALITY BIOLOGICAL, INC.

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes.

Supplier: QUALITY BIOLOGICAL, INC.

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Supplier: QUALITY BIOLOGICAL, INC.

MOPS buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane.

Supplier: QUALITY BIOLOGICAL, INC.

Penicillin-Streptomycin acts in two ways. Penicillin inhibits bacterial cell wall synthesis. While, streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Supplier: QUALITY BIOLOGICAL, INC.

Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Supplier: QUALITY BIOLOGICAL, INC.

Fischer’s medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD), for serial propagation of cells from leukemic mice. Fischer’s medium supports clonal growth of cells – particularly lymphoblasts – from primary explants or cells already in culture.

Supplier: QUALITY BIOLOGICAL, INC.

Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris hydrochloride provide convenient and highly purified solutions.

Supplier: QUALITY BIOLOGICAL, INC.

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution.

Supplier: QUALITY BIOLOGICAL, INC.

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt.

Supplier: QUALITY BIOLOGICAL, INC.

Ampicillin in a convenient ready-to-use solution useful in selecting for ampicillin resistant bacteria. Ampicillin is a β-lactam antibiotic with an amino group side chain attached to the penicillin structure.

Supplier: QUALITY BIOLOGICAL, INC.

Suitable for molecular biology, protein chemistry, and biochemical applications.

Supplier: QUALITY BIOLOGICAL, INC.

DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting.

Supplier: QUALITY BIOLOGICAL, INC.

Amphotericin B prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi.

Supplier: QUALITY BIOLOGICAL, INC.

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). Although, RPMI-1640 has been shown to support the serum-free growth of a number of cells.

Supplier: QUALITY BIOLOGICAL, INC.

Bisulfateless Brucella Plate (BLBB) is applicable for use in the primary isolation, quantitation, and partial identification of obligately anaerobic microorganisms from clinical specimens.

Supplier: QUALITY BIOLOGICAL, INC.

SOC medium is a ready-to-use solution, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli.

Supplier: QUALITY BIOLOGICAL, INC.

McCoy’s 5A Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. McCoy’s 5A Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Supplier: QUALITY BIOLOGICAL, INC.

MEM NEAA contains the same non-essential amino acids found in the standard MEM, at a strength of 100. MEM NEAA 100X can be used to enhance cell growth and prolong the viability of cells in culture. MEM NEAA 100X is commonly used as a supplement to minimal basal medium, and does not dilute the components already in the cell culture medium.