Supplier: CUBE BIOTECH

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Supplier: CUBE BIOTECH

The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: MP Biomedicals

Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: Thermo Scientific

Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

Supplier: Thermo Scientific

Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Supplier: Thermo Scientific

Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

Supplier: Thermo Scientific

Thermo Scientific Pierce SDAD (NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Thermo Scientific

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.

Supplier: Thermo Scientific

Thermo Scientific Pierce EMCH is a mid-length, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Supplier: Thermo Scientific

Thermo Scientific Pierce SDA (NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: Thermo Scientific

Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Supplier: Thermo Scientific

Thermo Scientific Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 angstroms).

Supplier: Thermo Scientific

Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Thermo Scientific

Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Supplier: MilliporeSigma

SMCC, Water-Soluble, a water-soluble derivative of SMCC.

Supplier: Thermo Scientific

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Supplier: Thermo Scientific

Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Supplier: Thermo Scientific

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Supplier: Thermo Scientific

Thermo Scientific Pierce Sulfo-SDA (Sulfo-NHS-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 3.9Å spacer arm separates the two photoreactive groups.

Supplier: AGILENT TECHNOLOGIES, INC (CSD) CA

Proven, fully porous column technology based on ZORBAX silica for reversed phase LC separations.

Supplier: Thermo Scientific

Thermo Scientific EZ-Link Hydrazide-PEG4-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.

Supplier: Thermo Scientific

Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Supplier: CUBE BIOTECH

Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

Supplier: Thermo Scientific

Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

Supplier: Thermo Scientific

Thermo Scientific Pierce LC-SDA (NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Supplier: Thermo Scientific

Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

Supplier: Thermo Scientific

Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Supplier: Thermo Scientific

Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

Supplier: Thermo Scientific

The Thermo Scientific Megafuge™ 8 centrifuge provides exceptional capacity in a compact design with a smart, simple interface and the flexibility to support both clinical and research applications. The centrifuge fits in to space, with its compact footprint, and to applications, accomodating multi-laboratory settings.