952 Results for: "New England Biolabs (NEB)"

Supplier: New England Biolabs (NEB)

This enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.

Supplier: New England Biolabs (NEB)

NEBNext® Enzymatic Methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.

Supplier: New England Biolabs (NEB)

Endoglycoceramidase I (EGCase I) catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids.

Supplier: New England Biolabs (NEB)

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers.

Supplier: New England Biolabs (NEB)

mRNA has emerged as a potent modality for therapeutics.

Supplier: New England Biolabs (NEB)

The Monarch spin PCR and DNA cleanup kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription.

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned and modified BtsI gene from Bacillus thermoglucosidasius (X. Pan).

Supplier: New England Biolabs (NEB)

Faustovirus Capping Enzyme (FCE) catalyzes the addition of N7-methylguanosine cap (m7G) to the 5' end of triphosphorylated and diphosphorylated transcripts, producing Cap-0 RNA. FCE is a single-subunit enzyme that combines the three activities necessary to produce the Cap-0 structure- triphosphatase, guanylyltransferase, and (guanine-N7)-methyltransferase. Installation of Cap-0 is a key step in eukaryotic mRNA maturation along with 2'-O-methylation at position 1 (Cap-1) and polyadenosine (poly(A)) tailing.

Supplier: New England Biolabs (NEB)

A DNA specific exonuclease isolated from Methylocaldum szegediense that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction with optimal activity between 45 and 60 °C. Isolated from a strain of E. coli which carries the cloned pMC45-1 gene from Methylocaldum szegediense.

Supplier: New England Biolabs (NEB)

Induro reverse transcriptase is a group II intron-encoded RT that exhibits increased thermostability, high processivity, and increased tolerance of inhibitors in the synthesis of cDNA from RNA.

Supplier: New England Biolabs (NEB)

The LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use.

Supplier: New England Biolabs (NEB)

The Control LAMP Primer Mix (rActin) targets actin RNA at an exon-exon junction. It is a LAMP primer set (F3, B3, FIP, BIP, LF, LB) that amplifies rActin to ensure the absence of inhibition from human nucleic acid templates in a LAMP reaction.

Supplier: New England Biolabs (NEB)

The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input, which is relevant for applications where RNA present in low abundance is of interest, such as pathogen detection.

Supplier: New England Biolabs (NEB)

The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step.

Supplier: New England Biolabs (NEB)

Quick-Load Purple 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye

Supplier: New England Biolabs (NEB)

The Monarch StabiLyse DNA/RNA Buffer is a dual-function reagent for nucleic acid stabilization and sample lysis.

Supplier: New England Biolabs (NEB)

9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Supplier: New England Biolabs (NEB)

β-N-Acetylglucosaminidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing β-N-Acetylglucosamine residues from oligosaccharides.

Supplier: New England Biolabs (NEB)

An E.coli strain that carries the cloned FatI gene from Flavobacterium aquatile NL3 (S.K. Degtyarev).

Supplier: New England Biolabs (NEB)

NsiI-HF is produced in E. coli from a strain that carries the clone and modified NsiI gene from Neisseria sicca (ATCC 29256).

Supplier: New England Biolabs (NEB)

E.coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).

Supplier: New England Biolabs (NEB)

A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA molecules, leaving blunt, ligatable ends.

Supplier: New England Biolabs (NEB)

E. coli RecA is necessary for genetic recombination, reactions involving DNA repair and UV-induced mutagenesis. RecA promotes the autodigestion of the lexA repressor, umuD protein and lambda repressor. Cleavage of LexA derepresses more than 20 genes. In vitro studies indicate that in the presence of ATP, RecA promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. The reaction has three distinct steps: (i) RecA polymerizes on the single-strand DNA, (ii) the nucleoprotein filament binds the duplex DNA and searches for a homologous region, (iii) the strands are exchanged.

Supplier: New England Biolabs (NEB)

The CpG Methyltransferase (M.SssI) methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5'...CG...3'.

Supplier: New England Biolabs (NEB)

Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys

Supplier: New England Biolabs (NEB)

The Blue Loading Buffer Pack contains 3X Blue Loading Buffer (8ml) and 30X Reducing Agent (1ml).

Supplier: New England Biolabs (NEB)

Anti-MBP Monoclonal Antibody is a murine anti-maltose-binding protein antibody, isotype IgG2a. It is purified from tissue culture supernatant by protein A affinity chromatography.

Supplier: New England Biolabs (NEB)

TriDye™ 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis

Supplier: New England Biolabs (NEB)

This primer is used for the priming and sequencing of mRNA adjacent to the 3'-poly A tail or tailed cDNA

Supplier: New England Biolabs (NEB)

TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis