Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: VWR

A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection.

Supplier: Cytiva

ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.

Supplier: Cytiva

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Supplier: Brady Worldwide

ToughWash® material polyester film with acrylic adhesive is a strong, print-on-demand label specially formulated for the Food and Beverage processing industry.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Supplier: Biotium

Commonly used fluorescent dyes for detection of oxidative activities in cells.

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins.

Supplier: Cytiva

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

Supplier: AAT BIOQUEST INC

Cy3-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labeled proteins in cells, solution and solid surfaces.

Supplier: AAT BIOQUEST INC

Cy5-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labeled proteins in cells, solution and solid surfaces.

Supplier: Biotium

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids.

Supplier: AAT BIOQUEST INC

iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labeled proteins in cells, solution and solid surfaces.

Supplier: Enzo Life Sciences

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.

Supplier: Enzo Life Sciences

The kit offers a set of six different MAbs specific for serine phosphorylation sites.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Enzo Life Sciences

Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

Supplier: Diagnostic Biosystems

SITVue /DAB detection system is a powerful Intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods.

Supplier: Biotium

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

Supplier: Biotium

These kits use enzymatic labeling of DNA double-strand breaks for fluorescence staining of apoptotic cells in fixed samples or tissue sections by TUNEL.

Supplier: Genetex

Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

Supplier: Biotium

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids.

Supplier: Thermo Scientific

Label peptides, antibodies and other proteins at primary amines with the coumarin-based, 405 laser-detectable Thermo Scientific DyLight™ Blue-emitting Specialty Dye

Supplier: Enzo Life Sciences

This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites.

Supplier: Enzo Life Sciences

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.

Supplier: Biotium

Digoxigenin-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3'-end terminal labeling to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. The digoxigenin (DIG) labeled probe could be detected using a labeled anti-digoxigenin antibody.