559 Results for: "Electrophoresis Buffers"

Supplier: MP Biomedicals

This 5X Direct Loading buffer contains a densifying agent and a red-purple dye that allow to follow the migration of the sample during the electrophoresis.

Supplier: G-Biosciences

G-Biosciences' FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

Supplier: Thermo Fisher Scientific

Horizontal systems for detailed RNA/DNA analysis deliver consistent, quality results

Supplier: VWR

Negatively charged anionic detergents are strong detergents utilized in lysis buffers for the complete disruption of membranes and denaturation of proteins. Anionic detergents are also used in denaturing gel electrophoresis.

Supplier: G-Biosciences

G-Biosciences GenLysate™ total protein lysates are ready to use for SDS-PAGE and Western blotting purposes.

Supplier: G-Biosciences

An effective proteome analysis requires the preparation of a sample to bring the wide range of protein species into the dynamic range of detection

Supplier: G-Biosciences

G-Biosciences' GenLysate™ total protein lysates are ready to use for SDS-PAGE and Western blotting purposes.

Supplier: Rockland Immunochemical

Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III

Supplier: G-Biosciences

2D-Xtract™ is a dry, urea-based and ready-to-use protein solubilization buffer.

Supplier: Edvotek

For centuries, humans have used selective breeding and conventional hybridization to produce desirable qualities and to increase crop yields. Today, scientists use genetic engineering to directly manipulate the DNA, quickly producing these desirable traits. In this experiment, students will use agarose gel electrophoresis to explore the molecular methods used by scientists to identify genetically modified organisms. No thermal cycler is required. Students are also encouraged to explore the controversy surrounding the use of genetically modified organisms.

Supplier: GROWCELLS

Tris (tris[hydroxymethyl]aminoethane) is widely used as a component of buffer solutions

Supplier: Microflex

Made from a patented MICROFORCE™ barrier SMS fabric that offers barrier protection against dry particulates and light liquid sprays.

Supplier: HARDY DIAGNOSTICS CA

CulGenex™ Heat-and-Pour™ Agarose Gels, which contain ethidium bromide, agarose, and with either TAE or TBE buffers, are recommended for agarose gel electrophoresis in the separation, visualization, quantification, or isolation of particular DNA fragments.

Supplier: MP Biomedicals

Product is the lyophilized powder of human secretory IgA and buffer salts.

Supplier: VWR

Ethidium Bromide solution is a fluorescent stain for visualizing nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0.625mg/mL. A final working concentration of 0.5g/mL is easily obtained by adding 1 drop of solution to 50 mL of molten agarose, running buffer or staining buffer. Ethidium Bromide solution in dropper bottles may be stored at room temperature for up to 1 year.

Supplier: Thermo Scientific Chemicals

Sodium acetate buffer solution pH, 5.2 is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis. Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as Chip-on-Chip assay and in vitro enzyme inhibition assay.

Supplier: AGILENT TECHNOLOGIES, INC (CSD) CA

PVA coated capillaries contain a permanently adsorbed layer of polyvinyl alcohol.

Supplier: VWR

A convenient pre-mixed solution that is easily diluted for buffer preparation under denaturing conditions.Commonly used to denature nucleic acids for electrophoresis and to study the secondary and tertiary structure of proteins. Effective for maintaining DNA in a highly denatured state during sequencing protocols, urea is also useful for accelerating the activity of Proteinase K digestions.

Supplier: MP Biomedicals

Lyophilized from 0.02M Ammonium Bicarbonate (may contain traces of buffer salt).
Immunological identity verified by radial immunodiffusion. Cellulose acetate electrophoresis shows one band indicating β2 electrophoretic mobility. SDS-PAGE shows one band corresponding to a molecular weight of approximately 12,000.
Reconstitution: To reconstitute use a phosphate buffer, pH >7.0, containing 0.15 M NaCl.

Supplier: Genscript

GenScript's Bis-Tris gels are high-performing polyacrylamide gels designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. Furthermore, the neutral pH which minimizes protein modification when compared to Tris-glycine gels.

Supplier: Cytiva

The key to reproducible electrophoresis results is temperature control. Gels cooled on their two faces produce slanted bands appearing as broad or double bands in stained gels. SE 600 Ruby ensures uniform heat in gels because, the gels are immersed in the lower buffer for uniform heat transfer and a standard magnetic stirrer circulates buffer around the vertically suspended heat exchanger to maintain homogeneous lower buffer temperature. The ergonomic SE 600 Ruby provides two built-in side handles, which facilitates carrying and handling. Run up to four gels (up to 112 samples) in a single run. Divider plates convert single-gel sandwiches into two-gel club sandwiches, which seal and fit into the casting stand like single gels. If gels longer than 16-cm are required, Cytiva offers the SE 660, which runs gels up to 24 cm. Except for gel lengt, the SE 660 has identical features to those of SE 600 Ruby.

Supplier: PROMEGA SUNNYVALE INC SE

Pre-mixed, and ready to use, this master mix contains DNA polymerase, dNTPs, MgCl₂, and reaction buffers at optimal concentrations for the amplification of DNA via PCR. Its special formulation allows for direct loading of PCR samples into agarose gels as well as tracking the migration during electrophoresis without the need for loading dyes. Simply add template, primers, and nuclease-free water, available separately. Mater Mix reduces risk of contamination. Includes enough for 100 reactions. Note: Can be stored at 4 °C for up to six weeks, or −20 °C for longer.

Supplier: IBI Scientific

Ultra Sieve Agarose is designed for high resolution electrophoresis where resolution of polyacrylamide gels are needed. Ultra Sieve Agarose improves clarity of gels and enhances visualization, even at high concentrations. Ultra Sieve Agarose has low viscosity, therefore high concentration gels can be made with high resolving capacity. The high gel strength, low concentration of 1.0-1.5% can also be made. Using TAE buffer, make a 1.8% gel for 400-1200bp, 3.0% gel for 150-800bp, and 4.5% gel for 15-400bp. To achieve the best resolution, store ultra sieve gels at +4-8°C for 30 minutes prior to use.

Supplier: Proteintech

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Supplier: VWR International

VWR® loading buffers, with three different dyes and migration fronts, make it easy to select the right loading buffer for your specific task.

Supplier: VWR

Loading Dye Base, 50X is used for custom loading buffer preparation.

Supplier: IBI Scientific

All models include buffer chamber, safety cover, power cords, casting system with tray, two combs, leveling bubble, and manual.

Supplier: Thermo Fisher Scientific

System features an extended upper buffer chamber that provides even heat distribution.

Supplier: Thermo Scientific Chemicals

6X DNA Loading Buffer is a pre-mixed solution used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.