"Cube Biotech"

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

Decylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Supplier: Cube Biotech

AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-55 - are highly-alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-55 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general, lighter AASTYs, like 6-55 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: Cube Biotech

PureCube Epoxy Agarose resin is your best option to couple biomolecules that carry free-standing amine or thiol groups.

Supplier: Cube Biotech

Our PureCube 100 Co-NTA agarose resins are agarose resin beads with a diameter of 100 µm. They are used for the purification of active His-tagged proteins from cells.

Supplier: Cube Biotech

Glyco-DIBMA is a modified version of DIBMA with a sugar side-chain added to the polymer molecule. Its purpose is to enhance DIBMA's range for solubilizing membrane proteins. After solving the Glyco-DIBMA + buffer powder in water, the product is ready-to-use. It can primarily be used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein of interest, the protein can be purified using affinity chromatography. We recommend the Rho1D4-tag for membrane protein purification and offer matching products for this purpose. DIBMA / Nanodisc Screening: Each membrane protein may perform better or differently with certain synthetic polymers. This is due to the characteristic composition of phospholipids surrounding the membrane proteins. Therefore a screening process for the correct DIBMA/Polymer is required in before. For this purpose, we offer our synthetic nanodisc screening kit MAXI which contains DIBMA, AASTY, and SMA.

Supplier: Cube Biotech

Membrane scaffold protein, human origin.

Supplier: Cube Biotech

AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-55 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-55 is named from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-55 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiencies, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: Cube Biotech

Elution of Rho1D4-tagged protein from matching purification beads.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

The HisCube His-tag protein purification MINI kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

Our PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

Supplier: Cube Biotech

Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Supplier: Cube Biotech

PureCube NHS (N-hydroxy succinimide) MagBeads/magnetic beads are your best option to couple biomolecules with free-standing amine groups.

Supplier: Cube Biotech

The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.