251 Results for: "CUBE BIOTECH"
PureCube Fe-NTA Agarose
Supplier: CUBE BIOTECH
PureCube Fe-NTA agarose resin beads were developed for the enrichment of phosphorylated proteins.
Expand 4 Items
N-Nonylphosphocholine ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.
Expand 4 Items
Nanodisc Rat MSP1E3D1-His DMPG
Supplier: CUBE BIOTECH
Membrane scaffold protein. Rat origin.
Expand 1 Items
Nanodisc MSP1E3D1-His DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Expand 1 Items
Anti-Strep Antibody
Supplier: CUBE BIOTECH
This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein.
Expand 1 Items
Anti-Strep Antibody (HRP (Horseradish Peroxidase))
Supplier: CUBE BIOTECH
This is an antibody specifically for targeting a Strep-(WSHPQFEK)-tag on a protein.
Expand 1 Items
DIBMA 12 in HEPES, PureCube for protein solubilisation
Supplier: CUBE BIOTECH
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.
Expand 3 Items
PureCube INDIGO Ni-MagBeads
Supplier: CUBE BIOTECH
PureCube Ni-INDIGO magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.
Expand 4 Items
N-Undecylphosphocholine ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.
Expand 3 Items
PureCube 100 NTA Agarose
Supplier: CUBE BIOTECH
PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 100 µm.
Expand 5 Items
PureCube NTA MagBeads
Supplier: CUBE BIOTECH
PureCube NTA (nitrilotriacetic acid) XL MagBeads were developed for IMAC-based protein purification and protein enrichment methods.
Expand 4 Items
PureCube 100 Compact Cartridge Co-NTA
Supplier: CUBE BIOTECH
The PureCube 100 Co-NTA compact cartridge is an IMAC chromatography column prepacked with PureCube 100 Co-NTA resin.
Expand 4 Items
Detergent Screening Set 2 (NG, NM, UDM, TDM, CHAPS), pure (> 99%)
Supplier: CUBE BIOTECH
Since for each protein, the optimal detergent needs to be screened, and because different detergents can be optimal for extraction, purification, and crystallization steps, Cube Biotech offers detergent screening sets containing small quantities of the most popular detergents at a special discounted price. All our detergents are of high quality, >99% pure, crystallization-grade.
Expand 1 Items
SMALP BZ25 Membrane protein solubilization & stabilization
Supplier: CUBE BIOTECH
The SMALP BZ series was developed in collaboration with Prof. Bert Klumperman from Nanosene, a spinout company of Stellenbosch University in South Africa. The goal was to create SMALPs that are synthesized via RAFT polymerization, achieving a better defined polymer size and a low dispersity (narrow chain length distribution). This is a significant improvement over previous SMALPs. The SMALP BZs are thus more efficient in dissolving the cell membranes and increasing protein yield.
After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Expand 3 Items
SMALP 300
Supplier: CUBE BIOTECH
The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.
Expand 3 Items
PureCube Co-NTA MagBeads XL
Supplier: CUBE BIOTECH
PureCube Co-NTA XL magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.
Expand 3 Items
PureCube Maleimide Activated Agarose
Supplier: CUBE BIOTECH
PureCube Maleimide Agarose resin is your best option to couple biomolecules that carry thiol (SH) groups.
Expand 5 Items
Nanodisc MSP1D1 DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Expand 1 Items
n-Nonyl-β-D-maltoside ≥99.5%, Crystallization grade
Supplier: CUBE BIOTECH
Mild, non-ionic detergents such as Nonylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.
Expand 3 Items
PureCube Glutathione MagBeads
Supplier: CUBE BIOTECH
The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.
Expand 4 Items
PureCube Al-NTA Agarose
Supplier: CUBE BIOTECH
PureCube Al-NTA agarose resin beads were developed for the enrichment of phosphorylated proteins.
Expand 4 Items
PureCube HiCap StrepTactin® MagBeads
Supplier: CUBE BIOTECH
PureCube StrepTactin® MagBeads are a suspension of 6% cross-linked magnetic agarose beads coupled to a StrepTactin protein.
Expand 4 Items
Nanodisc Mouse MSP2N2-His DMPC
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Expand 1 Items
PureCube 100 Compact Cartridge Ni-NTA
Supplier: CUBE BIOTECH
The PureCube 100 Ni-NTA compact cartridge is an IMAC chromatography column prepacked with PureCube 100 Ni-NTA resin.
Expand 4 Items
Nanodisc MSP1E3D1 DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Expand 1 Items
Nanodisc Mouse MSP1D1-His DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Expand 1 Items
Nanodisc MSP1E3D1-His POPC
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Expand 1 Items
Detergent Screening Set Classic (OG, LDAO, DM, DDM, OTG), pure (> 99%)
Supplier: CUBE BIOTECH
Since for each protein, the optimal detergent needs to be screened, and because different detergents can be optimal for extraction, purification, and crystallization steps, Cube Biotech offers detergent screening sets containing small quantities of the most popular detergents at a special discounted price. All our detergents are of high quality, >99% pure, crystallization-grade.
Expand 1 Items
PureCube 1-Step Batch MINI
Supplier: CUBE BIOTECH
Empty 1-step batch columns are ideally suited for batch purifications. They can be filled with your preferred affinity resin, allowing binding, washing, and elution in one spin column.
Expand 2 Items
Rat Recombinant MSP1D1-His (from E. coli)
Supplier: CUBE BIOTECH
Membrane scaffold protein, rat origin.