251 Results for: "CUBE BIOTECH"

Supplier: CUBE BIOTECH

Membrane proteins and their composition are as versatile as the whole cell membrane itself. For this reason, it is impossible to predict with 100% accuracy which SMALP product is best suited for the solubilization and subsequent preparation of your membrane protein of interest. We offer this SMALP Screening Kit that consists of our four individual SMAs. This way, you can conveniently screen for the best SMA for your protein before scaling up your assay. In case you would like to extend your polymer screening beyond the reaches of SMA, we offer our Synthetic Nanodisc Screening Kit, which covers both SMA and DIBMA.

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 dh5 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

PureCube NHS (N-hydroxy succinimide) MagBeads/magnetic beads are your best option to couple biomolecules with free-standing amine groups.

Supplier: CUBE BIOTECH

Sulfo-DIBMA is an electroneutral modification of existing DIBMAs. It does not interfere with charge-sensitive interactions between proteins and lipids. This innovation opens up a wider range of experimental research in terms of charge-sensitive membrane protein processes like protein-protein and protein-lipid interactions. In addition, Sulfo-DIBMA belongs to a new generation of DIBMA’s which are RAFT polymerized. This achieves a reduction in both monomer size and greater monodispersity. With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA is the lack of an absorbance maxima at 280 nm. SMAs, in comparison, usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.
Another significant advantage of Sulfo polymers compared to other polymers is the wide pH range in which they remain stable. The buffer in which the polymer is supplied has a pH of 7.5, but the polymer itself remains stable between pH 4 and pH 10. The special physicochemical properties of Sulfo-DIBMAs make them ideal for cryo-TEM and other downstream applications.
Good publications to find details about Sulfo-DIBMA and Sulfo-SMA are:
Oluwole et al. (2017)
Glueck et al. (2022)
Janson et al. (2022)
Eggenreich et al. (2023)

Supplier: CUBE BIOTECH

CHAPS is a zwitterionic detergent and its name is an abbreviation for 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Detergents are the most commonly used substances for membrane protein stabilization. Before you use a specific detergent for a new membrane protein you must screen for a matching one.

Supplier: CUBE BIOTECH

PureCube Cu-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: CUBE BIOTECH

PureCube IDA (iminodiacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

Supplier: CUBE BIOTECH

PureCube NHS (N-hydroxy succinimide) MagBeads/magnetic beads XL are your best option to couple biomolecules carrying free-standing amine groups.

Supplier: CUBE BIOTECH

DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Supplier: CUBE BIOTECH

Since for each protein, the optimal detergent needs to be screened, and because different detergents can be optimal for extraction, purification, and crystallization steps, Cube Biotech offers detergent screening sets containing small quantities of the most popular detergents at a special discounted price. All our detergents are of high quality, >99% pure, crystallization-grade.

Supplier: CUBE BIOTECH

Membrane scaffold protein, rat origin.

Supplier: CUBE BIOTECH

LDAO is one of the classic detergents that we offer in crystallization-grade quality at an attractive price. We aliquot our detergents so that they are convenient to use, keep fresh and provide optimal performance.

Supplier: CUBE BIOTECH

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum.

Supplier: CUBE BIOTECH

Synthetic copolymers are capable of solubilizing and stabilizing membrane proteins without the need for detergents. They have the unique capacity to extract the membrane protein of interest while maintaining a near-native environment.

Supplier: CUBE BIOTECH

Mild, non-ionic detergents such as Undecylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Supplier: CUBE BIOTECH

AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-45 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-45 gets it's name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

Nonylglucoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10, as well as octylthioglucoside.

Supplier: CUBE BIOTECH

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP2N2 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

Supplier: CUBE BIOTECH

Our E. coli cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Supplier: CUBE BIOTECH

Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Membrane scaffold protein, alpaca origin.

Supplier: CUBE BIOTECH

Sensory rhodopsin-2 (SR-2) is a 7-transmembrane helice protein that acts as a phototaxis receptor in archaebacteria.

Supplier: CUBE BIOTECH

The SMALP products have been developed for biomedical purposes, in particular, for the solubilization of (biological) lipid membranes and isolation & purification of integral membrane proteins.
After solving the SMA + buffer powder in water, the product is ready-to-use. It can primarily used for all cell membrane hosts, including bacteria, yeast, and human cells. After successful solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommend using the Rho1D4-tag. Cube Biotech offers matching products for this purpose.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

PureCube maleimide agarose XL resin is your best option to couple biomolecules carrying thiol (SH) groups.

Supplier: CUBE BIOTECH

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.

Supplier: CUBE BIOTECH

Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Supplier: CUBE BIOTECH

The Ultrasolute family evolved from the first amphipol A8-35 is used for highly efficient solubilization and stabilization of membrane proteins without the use of detergents while preserving their activity. They demonstrate tolerance to moderate concentrations of divalent cations and exhibit no absorption at wavelengths >250 nm.