Supplier: Anaspec Inc

This kit is optimized to detect anti-human MOG (1-125) IgG in human samples. Wells are pre-coated with recombinant human MOG (1-125) protein and pre-blocked with proprietary solution. The amount of anti- human MOG IgG in serum or plasma is quantified using ELISA. Human anti-Human MOG (1-125) standard is included. Ample materials and reagents are provided to perform 96 assays.

Supplier: Anaspec Inc

p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases

Supplier: Anaspec Inc

Beta-Amyloid (1-42) peptide, a major component of amyloid plaques, accumulates in neurons of Alzheimer’s disease brains. Biochemical analysis of the amyloid peptides isolated from Alzheimer’s disease brain indicates that Beta-Amyloid (1-42) is the principal species associated with senile plaque amyloids, while Beta-Amyloid (1-40) is more abundant in cerebrovascular amyloid deposit.
Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Molecular Weight: 4514.1 Da
% Peak Area by HPLC: ≥95
Peptide Content: ≥ 60%
Storage condition: -20°C

Supplier: Anaspec Inc

An H-2b-restricted OVA class II epitope.
Sequence: ISQAVHAAHAEINEAGR
MW: 1773.9 Da
% Peak Area by HPLC: ≥ 95%
Storage condition: -20°C

Supplier: Anaspec Inc

The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL®520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate. Ample materials are provided to perform 96 assays in a 96-well format.

Supplier: Anaspec Inc

The SensoLyte® Plus 520 MMP-13 Assay Kit is designed for specifically detecting MMP-13 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-13 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL®520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-13-induced cleavage of the FRET substrate. Ample materials are provided to perform 96 assays in a 96-well format.

Supplier: Anaspec Inc

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components

Supplier: Anaspec Inc

MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens

Supplier: Anaspec Inc

MMP-7 (matrilysin, PUMP) is involved in biological events, such as tissue remodeling, cell proliferation and host defenses.

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In Alheimer's Disease brains, plaques contain amino-terminal truncated beta-Amyloid peptides including the alpha secretase-generated p3 fragments, beta-Amyloid 17-40 and 17-42. They were shown to induce pro-inflammatory cytokine and chemokine production in vitro and in vivo.

Supplier: Anaspec Inc

This sequence corresponds to amino acids 3-13 from murine MBP. MBP induces immune reactivity in multiple sclerosis (MS), a chronic inflammatory demyelinating disease of the CNS. MBP (3-13) is a substrate for Protein Kinase C.
Sequence:QKRPSQRSKYL
MW:1390.6 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec Inc

PET (Positron Emission Tomography) imaging of [Lys3]-bombesin is able to detect gastrin-releasing peptide receptor (GRPR) positive prostate cancer. An immunoconjugate of [Lys3]-bombesin and corresponding monoclonal antibody can specifically induce (CD64)-dependent monocyte and neutrophil-mediated lysis of small cell carcinoma.
Sequence:Pyr-QKLGNQWAVGHLM-NH2
MW:1591.9 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec Inc

5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates.

Supplier: Anaspec Inc

HiLyte™ Fluor 488 C2 maleimide is an excellent thiol-reactive fluorescent labeling dye that generates the protein conjugates far superior to those of fluorescein derivatives such as FITC.

Supplier: Anaspec Inc

HiLyte™ Fluor 488 amine is a carbonyl-reactive fluorescent labeling dye.

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Environment-sensitive dye for studying membrane and protein structures

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Widely used for measuring membrane potential of mitochondria; 585/520 Fluorescence ratio increases upon cell hyper-polarization.

Supplier: Anaspec Inc

Despite the availability of alternative amine-reactive fluorescein derivatives that yield conjugates with superior stability and comparable spectra, fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. It appears that 5-FITC is more widely used than the 6-FITC isomer. FITC reagents are prominently used to label proteins. In addition, FITC has also been used to label peptides, oligonucleotides and other small organic ligands. A collection of recent applications is listed in the following references.

Supplier: Anaspec Inc

Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins

Supplier: Anaspec Inc

Urokinase-type plasminogen activator (uPA) is a serine protease that functions in the conversion of the zymogen plasminogen to the active, broad-spectrum serine protease plasmin

Supplier: Anaspec Inc

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, produced in the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels, leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is proposed as a therapeutic target for this disease.

Recombinant rat pro-renin was expressed in HEK cells. Purified enzyme was converted to the active renin by tryptic activation followed by removal of trypsin. The molecular mass of active rat renin is approximately 40 kDa. The activity of enzyme can be measured in FRET-based assays

Supplier: Anaspec Inc

The AnaTag™ B-PE Labeling Kit is optimized for use in the conjugation of B-Phycoerythrin (B-PE) to antibodies. B-PE, a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm.

Supplier: Anaspec Inc

Spectra of HiLyte™ Fluor 647 conjugates are slightly red-shifted compared to those of Cy5 dyes, resulting in an optimal match to filters designed for Cy5 dyes.

Supplier: Anaspec Inc

6-HEX is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research. It can also be used to label peptides and oligonucleotides.

Supplier: Anaspec Inc

QXL® 570 dyes are optimized quenchers for rhodamines (such as TAMRA, sulforhodamine B, ROX) and Cy3 fluorophores.

Supplier: Anaspec Inc

2,3-Naphthalenedialdehyde (NDA) ≥95% (by HPLC), Ultra Pure Grade

Supplier: Anaspec Inc

A substrate for DNA-dependent protein kinase (DNA-PK), phosphorylation. DNA-PK is essential for the repair of DNA double-strand breaks. This peptide corresponding to 11–24 amino acids of human p53 with threonine 18 and serine 20 changed to alanine is used as a substrate for the assay of DNA-PK activity
Sequence:EPPLSQEAFADLWKK
MW:1759 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec Inc

This product contains 0.03 mg (net) of the 32 CEF peptides for a total of 1 mg in one vial. Used in the stimulation of IFNγ release from CD8+ T cells in individuals with defined HLA types, these epitopes are useful in applications such as ELISPOT, intracellular cytokine and CTL assays.
% peak area by HPLC: ≥ 95
Storage condition: -20°C

Supplier: Anaspec Inc

This glycopeptide is an N-acetyl galactosamine (GalNAc)-modified MUC5AC mucin peptide containing the single site of threonine 13 labeled with GalNAc (T*). Polypeptide N-acetylgalactosaminyltransferase (ppGaNTase) catalyzes the transfer of GalNAc from the nucleotide sugar UDP-GalNAc to threonine. The MUC5AC gene is mainly expressed in gastric and tracheo-bronchial mucosae, and some tumors.
Sequence:GTTPSPVPTTST-T*-SAP (* = GalNAc-modified residue)
MW:1704.6 Da
% peak area by HPLC:95
Storage condition:-20° C

Supplier: Anaspec Inc

This peptide is histone H4, amino acids 1 to 21. It is acetylated at Lys-8 and at the N-terminus with a C-terminus GG linker, followed by a biotinylated lysine. The acetylation of histone H4 causes structural changes that amplify the binding of transcription factors to their recognition sites within the nucleosome.
Sequence:Ac-SGRGKGG-K(Ac)-GLGKGGAKRHRKV-GGK(Biotin)
MW:2644.1 Da
% peak area by HPLC:95
Storage condition:-20° C