352 Results for: ""Plasmid" purification systems"

Supplier: Beckman Coulter

A SPRI paramagnetic bead-based plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated without sacrificing product quality.

Supplier: Corning

AxyPrep™ Mag plasmid purification Kit utilizes a magnetic bead technology for high throughput purification of plasmid DNA from E.coli cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralization, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

Supplier: Proteintech

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Supplier: VWR International

ExoCleanUp FAST PCR reagent is a one-step PCR clean-up reagent for optimal sequencing results, consisting of a balanced combination of a heat-labile exonuclease I (HL-ExoI) and a recombinant shrimp alkaline phosphatase (rSAP).

Supplier: VWR

Fluorescent DNA Quantitation Kit uses the fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA

Supplier: Omega Bio-Tek

The E.Z.N.A.® Plasmid Maxi Kit is a maxi-scale system designed to deliver high-quality plasmid DNA using the spin column format, without costly accessories

Supplier: Zymo Research

Part of the ZymoPURE™ plasmid kits collection, the ZymoPURE™ plasmid miniprep kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes.

Supplier: Zymo Research

The Zyppy™ Plasmid Purification Kits feature a pellet-free procedure for the fastest purification of plasmid DNA.

Supplier: G-Biosciences

G­Biosciences' Toothpick™ systems allow for the rapid screening of bacterial colonies by restriction enzyme digestion analysis or polymerase chain reaction (PCR), with our Plasmid Screening Toothpick™ and Toothpick™­PCR, respectively.

Supplier: Zymo Research

The Zymoprep™ Yeast Plasmid Miniprep II used for plasmid isolation from S. pombe.

Supplier: New England Biolabs (NEB)

The NEBExpress Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17 to 230 kDa in just 2 to 4 hours under the control of T7 RNA Polymerase.

Supplier: Corning

The AxyPrep Mag Plant genomic DNA kit is a magnetic beads based purification system that allows rapid and reliable isolation of high quality gDNA from multiple plant samples

Supplier: G-Biosciences

G-Biosciences' GET™ Plasmid Miniprep kit isolates high quality plasmid DNA from 1-5 mL E

Supplier: Zymo Research

Simple DNA purification from a broad spectrum of fungal species.

Supplier: Zymo Research

Part of the ZymoPURE plasmid kits collection, the ZymoPURE II™ plasmid midiprep kits provides the fastest and simplest method available to efficiently isolate up to 1.2 of transfection grade plasmid DNA from E. coli.

Supplier: Omega Bio-Tek

Pre-scripted solution for the purification of cfDNA from up to 4 ml plasma samples.

Supplier: IBI Scientific

IBI Plant Isolate Total DNA Extraction Reagent System provides a quick and easy 3 step CTAB and chloroform based method to isolate Total DNA (including genomic, mitochondrial, and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria)

Supplier: Zymo Research

Part of the ZymoPURE plasmid kits collection, the ZymoPURE II™ plasmid gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 25 mg of transfection grade plasmid DNA from E. coli.

Supplier: Zymo Research

Prepare robust methyl-seq libraries in as little as three steps.

Supplier: IBI Scientific

IBI’s Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures

Supplier: Zymo Research

Part of the ZymoPURE plasmid kits collection, the ZymoPURE II™ plasmid maxiprep kits provides the fastest and simplest method available to efficiently isolate up to 3 mg of transfection grade plasmid DNA from E. coli.

Supplier: Omega Bio-Tek

Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

Supplier: Zymo Research

The Quick-RNA™ viral kit is a quick, purification system for viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield™.

Supplier: IBI Scientific

IBI's Midi Fast-Ion Plasmid Kits are endotoxin free and use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures

Supplier: Corning

The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

Supplier: ABCAM INC CA

ab185907 is designed to carry out bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina® platform-based bisulfite sequencing, all in one kit.

Supplier: Omega Bio-Tek

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources

Supplier: Omega Bio-Tek

The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections

Supplier: Beckman Coulter

This prep system kit isolates and purifies genomic DNA (gDNA) from whole blood. The kit is uses Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology to effectively produce a high recovery of high quality DNA for downstream applications such as PCR.

Supplier: ABCAM INC CA

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).