PureCube 1-Step Batch MIDI Plus

Empty 1-step batch columns are ideally suited for batch purifications. They can be filled with your preferred affinity resin, allowing binding, washing, and elution in one spin column.

• Usage: Specific binding and purification of proteins
• Specificity: Depending on the affinity of the used agarose resin
• Binding capacity: Also depending on the used agarose
• Body material: Polypropylene
• Filter pore size: 0.1 to 0.2 µm low binding PVDF
• Max. volume: 20 ml (midi)
• Max. g force: 1500 to 2000×g (swing-bucket rotor)
• Min. g force: 100 to 200×g for 1 to 10 min

Specifically designed for small to mid-scale protein purifications, PureCube 1-step batch spin columns save time and pipetting steps. Containing a SelfSeal membrane technology, the column retains resin and sample in a chamber for batch incubation. By centrifugation, the membrane pores dilate, and the filtered eluate gathers in the collection chamber of the column. PureCube 1-step batch spin columns are available in two sizes: Mini for expression trials, small-scale screening, and other small-volume purification needs or Midi Plus for up to 20 ml volumes.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and micropipetting tips
-50 ml conical tubes (e.g. Falcon)
- pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment optional: Western blot reagents and equipment