DNA denaturing solution

DNA Denaturing Solution is useful for denaturing fractionated DNA prior to the transfer from an electrophoresis gel to nitrocellulose or nylon membrane, as well as Northern and Southern blotting.

• Contains: 0.5 M NaOH and 1.5 M NaCl
• Filtered through a 0.22 µm membrane
• Dnase tested

The Denaturing Solution is a strong base, 0.5 M NaOH with 1.5 M NaCl, that denatures double-stranded DNA, so that it can be efficiently transferred to a nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe.

When utilized in conjunction with a depurination solution, treatment with the denaturing solution results in the hydrolysis of the phosphodiester backbone at the sites of depurination. This allows for a more efficient transfer of large DNA in neutral Southern transfers. Following DNA denaturation, the gel must be neutralized by soaking in DNA Neutralization Solution.

Sterile Filtered
UNSPSC: 41116126
Harmonization Code: 3822.00.5090
ECCN: EAR99
Max Shelf Life: 36 months

Certifications: Manufactured under a cGMP compliant quality management system in an ISO 9001:2015 certified facility.

Caution: Research Use Only