These non-ionic detergents are suitable for isolating membrane-protein complexes.
Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure. G-Biosciences proteomic grade detergent solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50 µS conductivity. These detergents are offered as sterile, 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications.
CAS Number: 9002-93-1
Molecular formula: C34H62O11 for x=10
Molecular weight: 647 (for x=10)
Absorbance (254 nm): 0.16 (0.05% w/v)
Critical micelle concentration (CMC): ~0.2 × 10 to 3M (25 °C)
Aggregation number: 100 to 155
Cloud point: 65 °C
Average micellar weight: 80000
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