Human Recombinant CCL4 (MIP-1B) (from E. coli)

Recombinant Human CCL4 (MIP-1B) in lyopholized format with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

: Larger sizes available, please request a custom quote.

: Shipped lyophilized at room temperature.

: For research use only, not for use in humans.

Conjugation:
Unconjugated
Protein Function:
Chemokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
Avoid repeated freeze-thaw cycles 12 months from date of receipt, –20...–70 °C as supplied. 1 month, 2...8 °C under sterile conditions after reconstitution. 3 months, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological activity:
EC50 = 0.23 nM determined by Migration Assay in cells expressing recombinant CCR5
Gene ID:
Accession # P13236 (24-92)
Reconstitution instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-free:
Y
Protease-free:
Y
Protein synonyms:
ACT2|AT744.1|G-26|HC21|LAG-1|LAG1|MIP-1-beta|MIP1B|MIP1B1|SCYA2|SCYA4
Protein/peptide name:
CCL4 (MIP-1B)
Purity:
>97%
Molecular weight:
7.82 kDa
Sequence:
APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN
Endotoxin level:
Very low
Formulation:
Lyophilized pellet
Shipping temperature:
RT
Tested applications:
Migration assay