Human Recombinant CCL2 (MCP-1) (from E. coli)

Supplier: CHEMOTACTICS, INC MS

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Supplier: CHEMOTACTICS, INC MS

About this item

Recombinant Human CCL2 (MCP-1) in lyopholized format with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

Monocyte chemoattractant protein-1 (MCP-1) (CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection.

Ordering information: Larger sizes available, please request a custom quote.

Delivery: Shipped lyophilized at room temperature.

Caution: For research use only, not for use in humans.

Specifications

Conjugation:
Unconjugated
Protein Function:
Chemokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
Avoid repeated freeze-thaw cycles 12 months from date of receipt, –20...–70 °C as supplied. 1 month, 2...8 °C under sterile conditions after reconstitution. 3 months, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological activity:
EC50 = 0.067 nM determined by Migration Assay of recombinant CCR2 containing cells
Gene ID:
Accession # P13500 (24-99)
Reconstitution instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-free:
Y
Protease-free:
Y
Protein synonyms:
HC11|MCAF|MCP1|MCP-1|SCYA2|GDCF-2|SMC-CF|HSMCR30
Protein/peptide name:
CCL2 (MCP-1)
Purity:
>97%
Molecular weight:
8.66 kDa
Sequence:
QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT
Endotoxin level:
Very low
Formulation:
Lyophilized pellet
Shipping temperature:
RT
Tested applications:
Migration assay