Human Recombinant CCL3 (MIP-1a) (from E. coli), Biotinylated

Supplier: CHEMOTACTICS, INC MS

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Supplier: CHEMOTACTICS, INC MS

About this item

Functional biotin labeled human CCL3 chemokine with very low endotoxin levels.

Extremely low endotoxin levels (<0.01 EU per 1 μg) Functional activity tested and validated For use in various streptavidin applications: Flow cytometry, microscopy, and other imaging techniques

Macrophage inflammatory proteins 1-α (MIP1α)(CCL3) binds to cell surface receptors CCR1 and CCR5. It is proinflammatory, leading to chemotaxis and activation of immune cells. It also inhibits proliferation of hematopoietic stem cells. Moreover, by binding to one of the HIV coreceptors, CCR5, it suppresses HIV infection.

Ordering information: Larger sizes available, please request a custom quote.

Delivery: Shipped lyophilized at room temperature.

Caution: For research use only, not for use in humans.

Specifications

Conjugation:
Biotin
Protein Function:
Chemokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
Avoid repeated freeze-thaw cycles. 12 months from date of receipt, –20... –70 °C as supplied. ChemoTactics suggests using immediately after reconstitution. 1 month, –20...–70 °C under sterile conditions after reconstitution.
Endotoxin content:
<0.01 EU per 1 μg of the protein by the LAL method
Biological activity:
EC50 = 1.1 - 2.6 nM determined by Migration Assay of recombinant CCR5 containing cells
Gene ID:
Accession # P10147 (24-92)
Reconstitution instructions:
Spin sample prior to reconstitution. Recommended concentration of 100 μg/ml in sterile water.
Endotoxin-free:
Y
Carrier-free:
Y
Protease-free:
Y
Protein synonyms:
G0S19-1|LD78|LD78ALPHA|MIP-1-alpha|MIP1A|SCI|SCYA3
Protein/peptide name:
CCL3 (MIP-1a)
Purity:
>97%
Molecular weight:
10.14 kDa
Endotoxin level:
Very low
Formulation:
Lyophilized pellet
Shipping temperature:
RT
Tested applications:
Migration assay