This kit is developed for serologic test for human IgM titer of Anti-SARS-CoV-2 Spike RBD (Wuhan Strain) antibody in serum/plasma or purified human antibody (monoclonal and polyclonal) in vitro. It is for research use only.
Antibodies of IgM and IgA class are a sign of an active infection (primary infection and reactivation) and disappear during convalescence. In some cases they can persist for several months.

• These kits were tested to bind to monoclonal antibodies in IgM format (P80H48) or human sera

• Recommendations – Customers who intend to use this kit to generate quantitative curves are suggested to consider the monoclonal antibodies to generate the reference curve: Recombinant Human Anti-SARS or SARS-CoV-2 S RBD IgM Antibody (P80H48).

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) caused the pandemic of COVID-19. The receptor-binding domain (RBD) subunit of the spike protein is to bind to its receptor on target cell surface (ACE2). A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 Spike RBD in human serum can facilitate research on antibodies produced in response to SARS-CoV-2 infection or vaccines.

Assay Principles: 

This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RBD subunit of Spike (Wuhan Strain) human IgM in serum. The kit consists of high-bind detachable 96-well plate pre-coated with Spike RBD protein, Positive control, Negative Control, an HRP-Anti-Human IgM secondary antibody, TMB and dilution/wash/stop buffer.

Use protocol (4 simple steps): 

1. Detach wells from plate based on your samples and keep at room temperature.  Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37 °C for 1 hour or room temperature for 1.5 hour. Wash wells 4 times by wash solution (0.3 ml/well).
2. Dilute the Secondary antibody HRP-Anti-Human IgM by wash solution and add to each well (0.1 ml/well). Incubate at room temperature for 0.5 - 1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml/well) and incubate at room temperature for 10 - 30 minutes. 
4. Stop the substrate reaction by adding stop solution (0.1 ml/well).  Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.

For research use only.