Human Neurofilament H ELISA kit (200 kDa Neurofilament protein) is a single-wash 90-min Simplestep used to quantify Human Neurofilament H (200 kDa Neurofilament protein) with a sensitivity of 141 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. Colorimetric Sandwich ELISA - 450 nm readout: works on any standard plate reader.