Ham's F12K (Kaighn's Nutrient Mixture F12) was originally developed for use in cloning Chinese hamster ovary and lung cells. This medium is useful with serum supplements for a variety of cell culture applications, including the growth of myeloma and hybridoma cells.
- Animal component-free
- Chemically defined
- GMP manufacture
With:
2500 mg/L Sodium bicarbonate
Without:
Ascorbic acid
Linoleic acid
L-Glutamine - add 10 ml/L (200 mM)
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- Calcium:Yes
- Magnesium:Yes
- HEPES:No
- Sodium bicarbonate:Yes
- Sodium pyruvate:Yes
- Phenol red:Yes
- Stable glutamine:No
- L-Glutamine:No
- Glucose level:Low
- Media Base:Ham's F12K (Kaighn's) Nutrient Mixture