You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Recombinant Streptavidin, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli
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Pierce™ PEGylated BM (bismaleimido) Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions
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Pierce™ GlycoLink™ Coupling Catalyst, Thermo Scientific
Supplier: Thermo Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
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SNAP-CELL® Block , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells
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SNAP-SURFACE® Block , New England Biolabs
Supplier: New England Biolabs (NEB)
SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells
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IMPACT™ Kit for Protein Purification, New England Biolabs
Supplier: New England Biolabs (NEB)
The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.
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Streptavidin, New England Biolabs
Supplier: New England Biolabs (NEB)
A 52.8 kDa non-glycosylated protein with a very high affinity for biotin
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Pierce™ Sulfhydryl Addition Kit, Thermo Scientific
Supplier: Thermo Scientific
Introduce free sulfhydryl groups into a protein of interest to allow the modified protein to react with a variety of sulfhydryl-selective products.
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Pierce™ DyLight™ Antibody Labeling, NHS Ester Quenchers, Thermo Scientific
Supplier: Thermo Scientific
DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes
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Chitin Resin, New England Biolabs
Supplier: New England Biolabs (NEB)
An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.
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BG-Maleimide, New England Biolabs
Supplier: New England Biolabs (NEB)
BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides
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Pierce™ CA(PEG)n Carboxy-PEG-Amine Compound, Thermo Scientific
Supplier: Thermo Scientific
The carboxy-PEGn-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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CLIP-Biotin, New England Biolabs
Supplier: New England Biolabs (NEB)
CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker
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DyLight™ Antibody Labeling, Long Stokes Shift Dyes, Thermo Scientific
Supplier: Thermo Scientific
The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.
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Pierce™ Immunostain Enhancer, Thermo Scientific
Supplier: Thermo Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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femtoCHROMO™- HRP Kit for Chromogenic Detection, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
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Pierce™ DyLight™ PEGylated Dyes, Antibody Labelling Dyes, Thermo Scientific
Supplier: Thermo Scientific
DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.
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HOOK™ Activated Agarose (Amine Reactive) for Affinity Column Generation, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups
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Pierce™ Pegylation Reagents, Methyl-PEG-Amine Compounds, Thermo Scientific
Supplier: Thermo Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers
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Template Switching RT Enzyme Mix, New England Biolabs
Supplier: New England Biolabs (NEB)
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template.
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Pierce™ ABC Peroxidase Standard Staining Kit, Thermo Scientific
Supplier: Thermo Scientific
Thermo Scientific Pierce ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers, Thermo Scientific
Supplier: Thermo Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.