You searched for: Protein Linking and Labeling Systems

Supplier: New England Biolabs (NEB)

SNAP-Surface Alexa Fluor 647 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells

Supplier: Thermo Scientific

The Thermo Scientific Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.

Supplier: G-Biosciences

G-Biosciences offers two different HOOK™ IgG Biotinylation Kits for labeling antibodies, either through free amines or sulfhydryls.

Supplier: New England Biolabs (NEB)

CLIP-Biotin is a cell-permeable substrate (BC-Biotin) based on biotin with an amidocaproyl linker

Supplier: Thermo Scientific

Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.

Supplier: New England Biolabs (NEB)

CLIP-Surface™ 547 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro

Supplier: Thermo Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Supplier: Thermo Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

Supplier: Thermo Scientific

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 - 145 nm in excitation/emission wavelengths.

Supplier: Thermo Scientific

The carboxy-PEG_n-amine (CA[PEG]n) PEGylation reagents are zwitterionic, amino acid derivatives that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers

Supplier: New England Biolabs (NEB)

BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as thiol-modified oligonucleotides

Supplier: G-Biosciences

G-Biosciences' CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

Supplier: G-Biosciences

G-Biosciences' Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm

Supplier: Thermo Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Thermo Scientific

The Thermo Scientific Pierce RNA 3'-End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.

Supplier: Thermo Scientific

DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes

Supplier: Thermo Scientific

DyLight™ Dyes have absorption spectra ranging from 350 nm to 770 nm and match the output wavelengths of common fluorescence instrumentation

Supplier: Thermo Scientific

Anti-TMT™ Antibody resin enables selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.

Supplier: Thermo Scientific

Thermo Scientific™ Pierce Fluorescence Biotin Quantitation Kit requires only ten microliters of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

Supplier: G-Biosciences

G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining

Supplier: New England Biolabs (NEB)

SNAP-Cell® Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag® in solution or in living cells

Supplier: New England Biolabs (NEB)

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.

Supplier: G-Biosciences

Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.

Supplier: G-Biosciences

G-Biosciences' amine reactive HOOK™ Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups

Supplier: G-Biosciences

G-Biosciences' avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates

Supplier: Thermo Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

Supplier: New England Biolabs (NEB)

SNAP-Surface® Block (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag® in solution or on the surface of living cells

Supplier: G-Biosciences

G-Biosciences' recombinant streptavidin comes from streptomyces avidinii, produced in E.coli

Supplier: New England Biolabs (NEB)

The IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) system is a novel protein purification system which utilizes the inducible self-cleavage activity of protein splicing elements (termed inteins) to separate the target protein from the affinity tag.

Supplier: G-Biosciences

G-Biosciences' HOOK™ BiotinQuant is designed for the estimation of biotin conjugation.