Unisol Amide stationary phase is made from high-purity spherical silica particles bonded with a neutral hydrophilic amide group.

• Metal Impurity: <30 ppm
• Pore Size: 100 Å
• Specific Surface Area: 410 m²/g
• Carbon Loading: 8 %
• Single End-capping
• pH range: 2.0 to 8.0

Unisol Amide columns offer strong retention of polar compounds in HILIC mode. They provide unique selectivity and are complementary to conventional reversed phase. Unisol Amide columns ensure more robust and reproducible performance than silica and amino stationary phase. They are 100% aqueous and 100% organic solvents compatible.

It can be used for normal phase, reversed phase, or hydrophilic interaction HPLC. The Unisol Amide (NP) column is a good replacement for NH₂ and silica columns that are currently marketed as HILIC. Compared with traditional silica and NH₂ columns, the Unisol Amide columns have better reproducibility and column lifetime.

It is especially useful for the separation of strong hydrophilic compounds, whether they are acidic, basic, or neutral. It is compatible with aqueous mobile phases in the pH range of 2.0 to 8.0. Unisol Amide presents unique selectivity for many molecules because of its difference in the separation mechanism from most of the conventional stationary phases.