NxGen™ M-MulV Reverse Transcriptase, Biosearch Technologies

Lucigen’s NxGen™ M-MulV Reverse Transcriptase is outstanding in cDNA synthesis.

M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Performance Specifications: 1) Unit Concentration: 200,000 U/mL. 2) Purity (SDS-PAGE): >99%. 3) SS Exonuclease: 2,000 U <5.0% released. 4) DS Exonuclease: 2,000 U <0.5% released. 5) Endonuclease: 2,000 U <10% converted. 5) E. coli 16S rDNA Contamination: 2,000 U <10 copies. 6) Storage: -20°C.

Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

: Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25°C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C. 250,000 U is supplied as 5 × 50,000 U. Unit definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are: