The Large DNA Fragment Extraction Kits are designed to recover large DNA fragments (>8Kb) from an agarose gel

The sample size for the Large kit is up to 300 mg of agarose gel. The sample size for the Small kit is 300 mg of agarose gel and up to 100 µL of PCR product.

The Small DNA Fragment Extraction Kits are designed to recover or concentrate small DNA fragments (50-200bp) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants are washed with a wash buffer (containing ethanol), and the purified DNA fragments are eluted by a low salt elution buffer or TE. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90% for the Small kit and 85% for the Large kit. The entire procedure can be completed in 45 minutes (Large) or 20 minutes (Small) and the DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling, and Ligation.