Optimizer Buffer™ For Optimal Cross-linking & Modification, G-Biosciences
About this item
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation
G-Biosciences has prepared six reaction-specific buffers that provide the optimal conditions for protein labeling, modification, and cross reaction. Optimizer Buffer™ -I is free of amines and is specific for amine reactive groups, such as imidoesters and N-hydroxysuccinimide (NHS) esters. Optimizer Buffer™-II has a high pH and is specifically designed for the sulfhydryl reactive haloacetyls, such as iodoacetyl. Optimizer Buffer™-III is designed for sulfhydryl reactive groups, maleimides and pyridyl disulfides, and has reagents that prevent oxidation of free sulfhydryls. Optimizer Buffer™-IV is designed to provide the optimal conditions necessary for carboxyl reactive reagents. Optimizer Buffer™-V is a modified acetate buffer that is specific for carbohydrate reactive groups. Optimizer Buffer™-VI is an alkaline buffer specific for the formation of Schiff’s base between amines and glyoxal groups.
G-Biosciences' buffers contain optimized concentrations of buffering agents, pH and other cofactors for specific reactions. Simply exchange the buffer of your sample with a suitable Optimizer Buffer™, and you are ready for efficient reaction. Use of G-Biosciences' SpinOUT™ or Tube-O-DIALYZER™ is recommended for buffer exchange and optimal reaction results. Each Optimizer Buffer™ is supplied as a 5X concentrated buffer.
Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
6 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition, for optimal conjugation
G-Biosciences has prepared six reaction-specific buffers that provide the optimal conditions for protein labeling, modification, and cross reaction. Optimizer Buffer™ -I is free of amines and is specific for amine reactive groups, such as imidoesters and N-hydroxysuccinimide (NHS) esters. Optimizer Buffer™-II has a high pH and is specifically designed for the sulfhydryl reactive haloacetyls, such as iodoacetyl. Optimizer Buffer™-III is designed for sulfhydryl reactive groups, maleimides and pyridyl disulfides, and has reagents that prevent oxidation of free sulfhydryls. Optimizer Buffer™-IV is designed to provide the optimal conditions necessary for carboxyl reactive reagents. Optimizer Buffer™-V is a modified acetate buffer that is specific for carbohydrate reactive groups. Optimizer Buffer™-VI is an alkaline buffer specific for the formation of Schiff’s base between amines and glyoxal groups.
G-Biosciences' buffers contain optimized concentrations of buffering agents, pH and other cofactors for specific reactions. Simply exchange the buffer of your sample with a suitable Optimizer Buffer™, and you are ready for efficient reaction. Use of G-Biosciences' SpinOUT™ or Tube-O-DIALYZER™ is recommended for buffer exchange and optimal reaction results. Each Optimizer Buffer™ is supplied as a 5X concentrated buffer.
Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
Documents
Product Family Options
Product Information
- Reactive groupTarget groupAvailabilityPrice
- CA82022-954Optimizer Buffer™-I [5X]Optimizer Buffer™-I [5X]NHS-ester & imidoester groupsAmine & PhotoreactiveLog in for availabilityPack of 2 (25ml)$551.53Supplier Number: BKC-04Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA82022-956Optimizer Buffer™-II [5X]Optimizer Buffer™-II [5X]Iodoacetyl groupsSulfhydrylLog in for availabilityPack of 2 (25ml)$551.53Supplier Number: BKC-05Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA82022-958Optimizer Buffer™-III [5X]Optimizer Buffer™-III [5X]Maleimides & pyridyl sulfidesSulfhydrylLog in for availabilityPack of 2 (25ml)$551.53Supplier Number: BKC-06Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA82022-960Optimizer Buffer™-IV [5X]Supplier Number: BKC-07Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA82022-962Optimizer Buffer™-V [5X]Optimizer Buffer™-V [5X]Hydrazide groupsCarbohydrateLog in for availabilityPack of 2 (25ml)$551.53Supplier Number: BKC-08Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- CA82022-964Optimizer Buffer™-VI [5X]Supplier Number: BKC-09Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
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Catalog #: CA21905-026
Supplier: Kimberly-Clark