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- Description:NI™ (Non-Interfering™) Protein Assay kit with Albumin Standard
- Size:500 Preps
- Environmentally Preferable:
- Cat. no.:CA82022-356
- Supplier No.:786-005
A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation
Universal Protein Precipitating Agent (UPPA™) is added to the protein solutions to rapidly precipitate total protein. Protein is immobilized by centrifugation and interfering agents in the supernatant are discarded. Protein concentration is assayed by mixing with an alkaline copper solution; the copper ions bind to the peptide backbone and the assay measures the unbound copper ions. The assay is independent of protein side chains minimizing protein-to-protein variation (see graph). The color density is inversely proportional to the amount of protein.
Has a linear response of 0.5 µg - 50 µg protein while only requiring 1-50µL of sample. Total protocol time for this product is around 30 minutes.
The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and other common laboratory items (see table). The NI™ Protein Assay is composed of two simple steps (see figure). A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and other common laboratory items (see table). The NI™ Protein Assay is composed of two simple steps (see figure).