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- Description:InGel™ Array
- Size:500 preps
- Cat. no.:CA82021-380
- Supplier No.:786-241A
G-Biosciences' InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments
The protein spots are first excised from the silver stained gel and transferred to a proteomic grade titer plate. Silver stained gel pieces are washed with G-Biosciences' SilverOUT™ to remove inhibitory silver ions. The proteins are then digested within the gel using a Mass Spectrometry Grade Trypsin and a supplied Trypsin Digestion Buffer.
The digested peptides are extracted with G-Biosciences' Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without any subsequent treatments or cleaning procedures.
G-Biosciences' InGel™ Array kit is supplied with reagents sufficient for up to 500 protein spots. The kit includes SilverOUT™ for the removal of silver ions, Trypsin Digestion Buffer for optimal trypsin activity, Pep-Extract™ for high level peptide extraction, and the InGel™ Array titer plates and caps. MSG-Trypsin™ (Mass Spectrometry Grade) trypsin, which increases stability, is also available but sold separately.
Fewer than 96 samples a batch may also be processed without any waste.