G-Biosciences' Superior™ Blocking Buffer is an enhanced blocking agent available in multiple formats

G-Biosciences' Superior™ Blocking Buffer is ideal for a high signal to background ratio in most systems and has a rapid blocking time of about two minutes for ELISA. Superior™ Blocking Buffer uses a non-serum protein and does not contain biotin or other animal source proteins that interfere with immuno-complexes. Superior™ Blocking Buffer is suitable for assays that use avidin/streptavidin systems.
G-Biosciences' Superior™ Blocking Buffer for Precipitating Substrate is a modification of the original product that has been optimized for use in blotting protocols that use precipitating substrates such as G-Biosciences' femtoCHROMO™ chromogenic detection systems, TMB (3, 3’, 5, 5’-Tetramethylbenzidine), BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrates. The Superior™ Blocking Buffer for Precipitating Substrate is not suitable for ELISA or immunohistochemistry staining.
G-Biosciences' Superior™ Blocking Buffer is available in multiple ready-to-use liquid formats: in TBS, PBS, TBS with 0.05% Tween® 20 detergent, or PBS with 0.05% Tween® 20. Also available is a convenient dry form that is stable at room temperature. Each dry buffer pack makes 200ml Superior™ Blocking Buffer (user is supplied with 5 packs).

Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.